Protein Info for Psest_1032 in Pseudomonas stutzeri RCH2

Annotation: penicillin-binding protein 1B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 770 transmembrane" amino acids 21 to 43 (23 residues), see Phobius details TIGR02071: penicillin-binding protein 1B" amino acids 24 to 746 (723 residues), 1009.1 bits, see alignment E=5.4e-308 PF14814: UB2H" amino acids 69 to 152 (84 residues), 84.8 bits, see alignment E=5.1e-28 PF00912: Transgly" amino acids 164 to 334 (171 residues), 203.7 bits, see alignment E=2.4e-64 PF00905: Transpeptidase" amino acids 426 to 666 (241 residues), 54.6 bits, see alignment E=1.6e-18

Best Hits

KEGG orthology group: K05365, penicillin-binding protein 1B [EC: 2.4.1.129 3.4.-.-] (inferred from 98% identity to psa:PST_3262)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJV9 at UniProt or InterPro

Protein Sequence (770 amino acids)

>Psest_1032 penicillin-binding protein 1B (Pseudomonas stutzeri RCH2)
MTKPRSPRTRSKRRSAGARPWLGWAVKLSIVGLVILAGFAIYLDAVVQEKFSGKRWTIPA
KVYARPLELFVGQKLDKNDFLAELDALGYRRERAVSGPGGASVSGNDIELHTRGFQFYEG
AEQSQRLRVRFSGDFVAGLSAGNGGTLPVARLEPVLIGGLYPAHNEDRILIKLDQVPPYL
VETLVAVEDREFFNHFGVSPKSIARAVWVNLTAGQVRQGGSTLTQQLVKNFYLTNERSLN
RKVTEAMMAVLLELHYDKQEILEAYLNEVFLGQDGRRAVHGFGLASQYFFGQPLAELKLP
QVALLVGMVKGPTYYNPRRNPERALERRNLVLDLLAEQQVVSADDVAKAKQAPLGVTQRG
SMADSSYPAFLDLVKRQLREDYRDEDLTEEGLRVFTSFDPILQLKAEQAMSETLKRLGKS
TEGVEGAMIVTNPETGEIQALLGSRQPGFAGFNRALDASRPIGSLIKPAIYLAALEKPSQ
YTLTSLLEDEPFSVKGADGQVWKPQNYGRQAYGTVYLYQALANSYNLSTARLGLDLGVPN
VLKTLERLGASPKWPAYPSMLLGAGGLRPIEVADMYQTLANGGFNTPLRGIRSVLTADGE
PLKRYPFQIQQRFDPGAIYLTQYAMQRAMSEGTGRSAYNRLPRSLNLAGKTGTTNDSRDS
WFAGFSQDLLAVVWLGRDDNGKTSLTGATGALQVWTDFMRRADPLPLQMPVPDNVTYAWV
DARSGLGTDERCPDAVQMPYIRGSEPAPGPGCGIQAPAESVMDWVRGWLQ