Protein Info for PGA1_c10160 in Phaeobacter inhibens DSM 17395

Annotation: amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 TIGR01891: amidohydrolase" amino acids 14 to 372 (359 residues), 337.5 bits, see alignment E=5.1e-105 PF01546: Peptidase_M20" amino acids 76 to 382 (307 residues), 160.9 bits, see alignment E=4.1e-51 PF07687: M20_dimer" amino acids 189 to 278 (90 residues), 27.9 bits, see alignment E=1.9e-10

Best Hits

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 72% identity to sil:SPO2810)

Predicted SEED Role

"N-acetyl-L,L-diaminopimelate deacetylase (EC 3.5.1.47)" in subsystem Lysine Biosynthesis DAP Pathway (EC 3.5.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.32 or 3.5.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DZ40 at UniProt or InterPro

Protein Sequence (388 amino acids)

>PGA1_c10160 amidohydrolase (Phaeobacter inhibens DSM 17395)
MPVKNRFAEMHAEITAWRRDIHQNPEILYETHRTSASVAEKLRTFGCDEVVTGIGRTGVV
GVIRGRSDTQNRAIGLRADMDALPMAEDTGLPHASQIEGAMHACGHDGHTAMLLGAAKYL
AETRNFDGTAVVIFQPAEEGGNGAEAMCKDGLMDRFGIDEVYAIHNSPGLELGKFALRPG
PILASVDEFIIRLQGRGGHAAKPQETVDTTVMMCQLITALQSIVSRNVDPVMQAVLSVTS
AETSSKAYNVIPDRAEVKGTIRTHSPDVRAHIPERLRAVTAGIALTFGGSAEVEFVNGVP
VTINDAEATGHAHAAALAVAGDCEEVALAMGGEDFSFMLEERPGAMIRVGNGSSAGLHHP
AYDFNDDAIPAGCSWFVTLIEQRLPLPR