Protein Info for Psest_1022 in Pseudomonas stutzeri RCH2

Annotation: Thiol:disulfide interchange protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 603 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 189 to 215 (27 residues), see Phobius details amino acids 228 to 252 (25 residues), see Phobius details amino acids 265 to 287 (23 residues), see Phobius details amino acids 309 to 338 (30 residues), see Phobius details amino acids 351 to 373 (23 residues), see Phobius details amino acids 381 to 399 (19 residues), see Phobius details amino acids 406 to 426 (21 residues), see Phobius details amino acids 438 to 457 (20 residues), see Phobius details PF11412: DsbD_N" amino acids 51 to 161 (111 residues), 103.6 bits, see alignment E=1.8e-33 PF02683: DsbD_TM" amino acids 186 to 403 (218 residues), 256.2 bits, see alignment E=5.1e-80 PF13386: DsbD_2" amino acids 192 to 396 (205 residues), 36.8 bits, see alignment E=9.7e-13 PF13899: Thioredoxin_7" amino acids 492 to 572 (81 residues), 59.9 bits, see alignment E=5.5e-20 PF13098: Thioredoxin_2" amino acids 501 to 594 (94 residues), 35.6 bits, see alignment E=2.6e-12

Best Hits

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 89% identity to psa:PST_3271)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJL9 at UniProt or InterPro

Protein Sequence (603 amino acids)

>Psest_1022 Thiol:disulfide interchange protein (Pseudomonas stutzeri RCH2)
MVIMPPQLYREIAMLRLLSLLFLLFVLPANAGLFDSKPSATLGGALNNSGDFLPVREAFR
LSLVEAAPERVTLRFVAADGYYLYRHRFAFQTSEPTVTLGEAKLPDGVPKVDDYFGEIEA
YYGIVDIEIPVTNPDNRPFVLQVSYQGCADKGLCYPPETESLRIGDGAESTTTTAGGQTS
QGDLSWKSIALFFLAGLGLTFTPCVLPMLPILTGVVLRDQPGGMRSFLLSLAYVLPMAGG
FALLGALMGVFGAELNLQARLQSPWVLVPFALFFVAFALAMFGLFELRLPQALASRLDRL
AGNARGGSFTGAALLGAVSSLLVSPCVSAPLAGALLYISASGDALGGGLKLFALGLGMGA
PLVLFATGGGALLPKSGPWMISVRNVFGVLLLAVAVWMLERVLPGPLALALWGLLAAGSA
VFLGTLEFTSKTPRQKLAQLAGLVLLVYALAAWVGALQGGSDPLRPLPHAIGGSTPSSMA
GEGWHTISTPAELDAQLAAARAAGQPLMLDWYADWCISCKVIEREVFANPQVAPRLADYR
LIRFDITESNAAQRSLLDRYKLFGPPAILFFDRSGNEMDDVRVVGEIDASGFAERLDRAA
AGL