Protein Info for PGA1_c01010 in Phaeobacter inhibens DSM 17395

Annotation: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase SpoT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 710 TIGR00691: RelA/SpoT family protein" amino acids 26 to 696 (671 residues), 695 bits, see alignment E=5.6e-213 PF13328: HD_4" amino acids 26 to 175 (150 residues), 168.1 bits, see alignment E=4e-53 PF01966: HD" amino acids 45 to 144 (100 residues), 37.7 bits, see alignment E=6.8e-13 PF04607: RelA_SpoT" amino acids 235 to 343 (109 residues), 144.7 bits, see alignment E=3.8e-46 PF02824: TGS" amino acids 389 to 447 (59 residues), 69.8 bits, see alignment 4.9e-23 PF19296: RelA_AH_RIS" amino acids 459 to 553 (95 residues), 70.1 bits, see alignment E=8.2e-23 amino acids 573 to 610 (38 residues), 27.8 bits, see alignment 8e-10 PF13291: ACT_4" amino acids 623 to 698 (76 residues), 55.4 bits, see alignment E=2.3e-18

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 90% identity to sit:TM1040_2565)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7ET68 at UniProt or InterPro

Protein Sequence (710 amino acids)

>PGA1_c01010 guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase SpoT (Phaeobacter inhibens DSM 17395)
MISPEDLIALVRNYNPRTNADRIADAYAFGEQMHDGQFRHSGEPYFTHPVAVAAILTEQR
LDDATIITALLHDTIEDTKASYDEISTRFGDEVAMLVDGVTKLTNLQLSSRETKQAENFR
KLFMAMSKDLRVILVKLADRLHNMRTIKAMRPDKQAQKARETMDIYAPLAGRMGMQWMRE
ELEDLAFRVLNPEGRQSIIRRFVTLQRETGDVIHRITGDMRAELEKTGIEAEVFGRAKKP
YSIWRKMQEKDQGFSRLSDIYGFRIITASEEDCYRALGAIHQRWRAVPGRFKDYISQPKS
NGYRSIHATVSGRDGKRVEVQIRTRQMHDVAETGVAAHWSYRDGVRSENPFAVDPAKWIA
SLTEQFDAEEDHDEFLEAVKLEMYSDQVFCFTPKGDVIKLPKGATPIDYAYAIHTRIGHA
CVGAKVDAIRVPLWTRLRNGQSVEIITAEGQTPQVSWLDIATTGKARTAIRRALREADRA
RFVKLGHELARSAFEHVGRKATDKALETAARALRVSTVDELLARLGAAVLTAHDVVQAVY
PELAADDSDEVSPRRAVIGLEPGQSFERAPCCQPLPGERIIGITYRGRGVVVHAADCDAL
GEVEDQPERWVDLHWHSGTHPAVYGVTLELTIGNDAGVLGRICTLIGEKKANISDLEFVD
RKPDFYRLMINVELRDVEQLHSLMLMLEAESDVAAVARFRDLPESRPKLG