Protein Info for PGA1_c09900 in Phaeobacter inhibens DSM 17395

Annotation: malate synthase GlcB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 7 to 708 (702 residues), 1066 bits, E=0
• PF20656: MS_N
  • amino acids 17 to 71 (55 residues), 41.1 bits, 2e-14
• PF20658: MSG_insertion
  • amino acids 157 to 224 (68 residues), 80.5 bits, E=1.7e-26
• PF01274: MS_TIM-barrel
  • amino acids 326 to 567 (242 residues), 212.5 bits, E=1.1e-66
• PF20659: MS_C
  • amino acids 579 to 681 (103 residues), 79.8 bits, E=4.6e-26

Best Hits

Swiss-Prot: 65% identical to MASZ_RHIME: Malate synthase G (glcB) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 84% identity to sit:TM1040_0711)

MetaCyc: 58% identical to malate synthase G (Mycobacterium tuberculosis H37Rv)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• glyoxylate cycle (5/6 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (4/7 steps found)
• L-arabinose degradation IV (4/8 steps found)
• D-xylose degradation IV (3/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (7/14 steps found)
• superpathway of pentose and pentitol degradation (11/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See I7DZ19 at UniProt or InterPro

Protein Sequence (710 amino acids)

>PGA1_c09900 malate synthase GlcB (Phaeobacter inhibens DSM 17395)
MSGMQERHDMQVADTLVSFIEDKALPGTGVTADAFWAGLAGLVNGMGDENRALLAKRADL
QGQIDAWHIERKGQAHDATAYEAFLRDIGYLLPEGDDFEIETQNVDDEIANVPGPQLVVP
ITNARFALNAANARWGSLYDALYGTDAMGDLPEGKGYDAGRGARVIAWGRGFLDQTFPLA
EGSWNDCVGLSVEGGALVPALKDAAQFAGYEGDAATPGKILLKNNGLHAVIVVDANGNIG
KGDQAGINDIVLESALSTIMDCEDSVACVDGEDKVTAYANWLGLMKRDLAEEVTKGGETF
TRVLNDDQTFTAPDGSDLVLKGRSLLLVRNVGHLMTNPAVRDSAGREAGEGFIDAMVTVL
CAMHDLQAEGGNSLHGSVYVVKPKMHGPEEVAFTDRIFTHVEDALGLPRHTVKIGIMDEE
RRTSVNLKSCIRAAKHRVAFINTGFLDRTGDEIHTSMEAGAMMRKGEMKSTPWIASYEDR
NVDIGLACGLKGRAQIGKGMWAMPDRMGEMLEAKIGHPKSGATCAWVPSPTAATLHATHY
HRVNVHEVQDQLKAGGARGTLDDLLTIPVMQGENLSDAEIAQEIENNAQGILGYVVRWVD
QGVGCSKVPDIHNVGLMEDRATCRISSQALANWLHHGIVDETQVMAAMQKMAAVVDGQNA
SDASYTPMAPGFDGIAFQAACDLVFRGRIQPSGYTEPVLHARRLELKAQG