Protein Info for PGA1_c09840 in Phaeobacter inhibens DSM 17395
Annotation: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 94% identical to GATB_RUEST: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (gatB) from Ruegeria sp. (strain TM1040)
KEGG orthology group: K02434, aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit B [EC: 6.3.5.6 6.3.5.7] (inferred from 94% identity to sit:TM1040_0701)MetaCyc: 43% identical to glutamyl-tRNAGln amidotransferase subunit B (Bacillus subtilis)
Glutaminyl-tRNA synthase (glutamine-hydrolyzing). [EC: 6.3.5.7]
Predicted SEED Role
"Aspartyl-tRNA(Asn) amidotransferase subunit B (EC 6.3.5.6) @ Glutamyl-tRNA(Gln) amidotransferase subunit B (EC 6.3.5.7)" (EC 6.3.5.6, EC 6.3.5.7)
MetaCyc Pathways
- L-citrulline biosynthesis (8/8 steps found)
- L-glutamate and L-glutamine biosynthesis (7/7 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.6, 6.3.5.7
Use Curated BLAST to search for 6.3.5.6 or 6.3.5.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See I7EKG9 at UniProt or InterPro
Protein Sequence (504 amino acids)
>PGA1_c09840 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (Phaeobacter inhibens DSM 17395) MLDLTYELPKPKVIAGAKHDWELVIGMEVHAQVSSNAKLFSGASTKFGAEPNSNVAFVDA AMPGMLPVINDYCVEQAVRTGLGLKADINLWSAFDRKNYFYPDLPQGYQISQLYHPIVGE GEVLVELGDGTARNVRVERIHMEQDAGKSIHDMDPNMSFVDLNRTGVCLMEIVSRPDIRG PEEAAAYIAKLRQIMQYLGTCDGNMQNGNLRADVNVSVCRPGQYEKYQETQDFSHLGTRC EIKNMNSMRFIQQAIDVEARRQIAIIEAGGTIDQETRLYDPDKGETRSMRSKEEAHDYRY FPDPDLLPLEIEQAWVDDIAANLPELPDEKKARFIKDFGLSDYDASVLTADVESATFFEQ TAKGRSGKLAANWVINELFGRLKKDEDKQITDSPVSPAQLGGIIDLISSDAISGKIAKDL FEIVYTEGGDPAQIVEERGMKQVTDTGAIEAALDEIIAANPAQVEKAKVNPKLAGWFVGQ VMKATGGKANPKAVNELVAKKLGE