Protein Info for PGA1_c09520 in Phaeobacter inhibens DSM 17395

Annotation: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase MurE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR01085: UDP-N-acetylmuramyl-tripeptide synthetase" amino acids 23 to 485 (463 residues), 430 bits, see alignment E=6.2e-133 PF01225: Mur_ligase" amino acids 24 to 102 (79 residues), 39.5 bits, see alignment E=8.8e-14 PF08245: Mur_ligase_M" amino acids 114 to 315 (202 residues), 166.3 bits, see alignment E=1.3e-52 PF02875: Mur_ligase_C" amino acids 336 to 421 (86 residues), 77.9 bits, see alignment E=9.1e-26

Best Hits

Swiss-Prot: 48% identical to MURE_ZYMMO: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K01928, UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase [EC: 6.3.2.13] (inferred from 80% identity to sit:TM1040_2018)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DNK5 at UniProt or InterPro

Protein Sequence (494 amino acids)

>PGA1_c09520 UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase MurE (Phaeobacter inhibens DSM 17395)
MSTIENRPLSQLGLTARGGVDPMIRGIAVDSRKVVEGGLFAALPGSQVHGAKFTPAALGN
GAAAILTDAAGAQIVAQLLRESTVALVVVEDPRQALAYAAALWFGAQPETMVAVTGTNGK
TSVASFVRQIWVELGHQAVNLGTTGVEGSWSHPLNHTTPEPVTLHDALAQAAAAGVTHAA
MEASSHGLEQRRLDGVHLAAAGFTNFTQDHLDYHETFEAYFTAKAGLFRRVLPDDGVAVI
NMDDPRGEQMRAVAAARGHEVISVGRGLGDLSLVGARYDASGQDMRFTWHDKPYQARLNL
IGGFQAENILLACGLVIASGEDPQAVFDTLPHLQTVRGRMQLAATRENGATVFVDYAHTP
DAVATAIKALRPHVLGRLVAIVGAGGDRDAAKRPLMGQAAQNNADLVIVTDDNPRSEDPA
VIRAAVKGGAPDAMEVGDRAEAILRGVDALGAGDALLICGKGHETGQIVGSDVLPFDDVE
QASMAVAVLDGRLV