Protein Info for HP15_906 in Marinobacter adhaerens HP15
Annotation: methylmalonate-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 73% identical to MMSA_PSEAE: Methylmalonate-semialdehyde dehydrogenase [acylating] (mmsA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K00140, methylmalonate-semialdehyde dehydrogenase [EC: 1.2.1.27] (inferred from 90% identity to maq:Maqu_2133)MetaCyc: 73% identical to methylmalonate-semialdehyde dehydrogenase subunit (Pseudomonas aeruginosa)
Methylmalonate-semialdehyde dehydrogenase (acylating). [EC: 1.2.1.27]
Predicted SEED Role
"Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Valine degradation (EC 1.2.1.27)
MetaCyc Pathways
- L-valine degradation I (7/8 steps found)
- β-alanine degradation I (1/2 steps found)
- β-alanine degradation II (1/2 steps found)
- propanoyl-CoA degradation II (3/5 steps found)
- 2,4-dinitrotoluene degradation (1/7 steps found)
- myo-inositol degradation I (1/7 steps found)
- myo-, chiro- and scyllo-inositol degradation (1/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.27
Use Curated BLAST to search for 1.2.1.27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PEW5 at UniProt or InterPro
Protein Sequence (497 amino acids)
>HP15_906 methylmalonate-semialdehyde dehydrogenase (Marinobacter adhaerens HP15) MKNVPLYIAGEFIQSQTKDWIEVTNPATNEVIAKAPNTTHAEMEQAIKYAGEVFKTWKEV PVSERARVMLRYQALLKKHHDEIAEILSQETGKTFEDAKGDVWRGIEVVEHAGNVASLMM GETVENVAREVDTHSWIQPLGVCAGITPFNFPAMIPLWMFPMAIACGNTFILKPSEQDPL TPMRLAELFEEAGAPKGVLQVVHGGKEQVDVLLTDPAIKAVSFVGSVPVGRYIYETGTRN MKRVQSFAGAKNHMVILPDADKQQVINALVGASVGAAGQRCMAISVAVFVGEAQQWIPEL KEAMAKVRPGAWNDSGASYGPIISAKAKDRIESLIATGEAQGANLLLDGRGCTVDGLPDG NWVGPTLFSGVTTEMDIYNEEIFGPVLSCMETDTLGDAIELVNNSPYGNGVSIFTSSGGA ARRFQHEIDVGQVGVNIPIPVPLPFFSFTGWKGSFYGDQHAYGKQAVRFYTETKTVTSRW FSSEATSSEANFSIQLR