Protein Info for PGA1_c09420 in Phaeobacter inhibens DSM 17395

Annotation: pyruvate carboxylase Pyc

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1188 PF00289: Biotin_carb_N" amino acids 46 to 155 (110 residues), 141.3 bits, see alignment E=8.5e-45 TIGR01235: pyruvate carboxylase" amino acids 48 to 1188 (1141 residues), 1805.1 bits, see alignment E=0 PF02786: CPSase_L_D2" amino acids 160 to 369 (210 residues), 231.1 bits, see alignment E=5.4e-72 PF07478: Dala_Dala_lig_C" amino acids 183 to 337 (155 residues), 32.6 bits, see alignment E=2.8e-11 PF02222: ATP-grasp" amino acids 184 to 339 (156 residues), 36.3 bits, see alignment E=2.4e-12 PF02785: Biotin_carb_C" amino acids 387 to 495 (109 residues), 109 bits, see alignment E=6.8e-35 PF00682: HMGL-like" amino acids 729 to 846 (118 residues), 54.2 bits, see alignment E=7.2e-18 PF02436: PYC_OADA" amino acids 870 to 1070 (201 residues), 250.8 bits, see alignment E=5.1e-78 PF00364: Biotin_lipoyl" amino acids 1121 to 1187 (67 residues), 49.5 bits, see alignment 1.5e-16 PF13533: Biotin_lipoyl_2" amino acids 1124 to 1154 (31 residues), 27 bits, see alignment (E = 1.5e-09)

Best Hits

KEGG orthology group: K01958, pyruvate carboxylase [EC: 6.4.1.1] (inferred from 66% identity to atu:Atu2726)

Predicted SEED Role

"Pyruvate carboxylase (EC 6.4.1.1)" (EC 6.4.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.4.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DNJ2 at UniProt or InterPro

Protein Sequence (1188 amino acids)

>PGA1_c09420 pyruvate carboxylase Pyc (Phaeobacter inhibens DSM 17395)
MRILPISGLRSALSRWPYPELRLYRTLICAYHFAKLHDPEDAMTDFKKILIANRGEIAIR
VMRAANEMGKKTVAVYAEEDKLGLHRFKADEAYRIGEGMGPVAAYLSIDEIIRVAKECGA
DAIHPGYGLLSENPDFVDACARNGITFIGPKAETMRALGDKASARRVAIDADVPVIPATE
VLGNDMDAIRKEAAEVGYPLMLKASWGGGGRGMRPIHSEDELEEKVLEGRREAEAAFGNG
EGYLEKMITRARHVEVQILGDKHGEIYHLYERDCSVQRRNQKVVERAPAPYLTEEQRTEI
CDLGRKICQHVNYECAGTVEFLMDMNDGKFYFIEVNPRVQVEHTVTEEVTGIDIVQAQIL
IAEGKTIAEATGKASQDEIQLNGHALQTRVTTEDPLNNFIPDYGRITAYRSATGMGIRLD
GGTAYAGGVITRYYDSLLTKVTAKAPTPEKAIARMDRALREFRVRGVSTNIAFVENLLKH
PTFLSNEYTTKFIDETPELFQFAKRRDRGTKVLTYIADISVNGHPETEGRAAPHTDLKEP
RAPKADAATQPYGTRNLLEQKGAQAVADWMKAQRQLLLTDTTMRDGHQSLLATRMRSHDM
IKVAPAYAQNLSQLFSVECWGGATFDVAYRFLQECPWQRLRDLRERMPNLMTQMLLRASN
GVGYTNYPDNVVQSFVKQAATGVDVFRVFDSLNWVENMRVAMDAVVESGKICEGTICYTG
DILDPNRAKYDLKYYVGMAKELEAAGAHILGLKDMAGLLKPAAARQLVKALKEEVGLPVH
FHTHDTSGVAGATILAAADAGVDAVDAAMDAFSGGTSQPCLGSIVEALRNTDRDTGIDIA
AVREISGYWEQVRAHYVAFESGLAAPASEVYLHEMPGGQFTNLKAQARSLGLEEKWSDVA
QTYADVNQMFGDIVKVTPSSKVVGDMALMMVSQGLTRDDVEDPKSDVAFPDSVVDMMRGN
LGQPPGGFPEAIVSKVLKGDAPNLERPGAHLAPVDLEATRAELSKELEGKDVDDEDLNGY
LMYPKVFLDYMGRHRTYGPVRSLPTRTFFYGMEPGEEITAEIDPGKTLEIRLQAIGDTDD
KGEVKVFFELNGQPRVIRVPNRLVKATTQANPKAEQGNPNHIGAPMPGVVASVAVQVGQP
VHEGDMLLTIEAMKMETGIHAERDAVVKAVHVQPGGQIDAKDLLIELE