Protein Info for HP15_898 in Marinobacter adhaerens HP15

Annotation: glutamine amidotransferase class-I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00117: GATase
  • amino acids 47 to 185 (139 residues), 74.6 bits, E=8.6e-25
• PF07722: Peptidase_C26
  • amino acids 78 to 183 (106 residues), 34.5 bits, E=1.9e-12

Best Hits

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 63% identity to maq:Maqu_2155)

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-glutamine degradation I (1/1 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Sequence Analysis Tools

See E4PEV7 at UniProt or InterPro

Protein Sequence (236 amino acids)

>HP15_898 glutamine amidotransferase class-I (Marinobacter adhaerens HP15)
MVVLKTGTTYPDIRARFGDFDEWFLRGLSDELDITVANAEAGELPGNPEDWDGIVVTGSP
AMVSDRAEWSENAGRWLVKAVRSEVPVLGVCYGHQLLAHALGGEAGYHPNGRETGTHEVE
LLPEAASDILFRELPEKFPAQLTHRQSVLRLPEGAVLLGRNQFEPHQAFRIGPCAWGVQF
HPEFTDAIMSAYLKVQAPDLTREGLDAEALIAGVKPAPDASSLLSRFSRFVQDRLR