Protein Info for HP15_886 in Marinobacter adhaerens HP15

Annotation: pseudouridine synthase family 1 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 312 PF01479: S4" amino acids 8 to 52 (45 residues), 52.7 bits, see alignment 2.9e-18 PF00849: PseudoU_synth_2" amino acids 69 to 194 (126 residues), 69.3 bits, see alignment E=4.5e-23 TIGR00093: pseudouridine synthase" amino acids 73 to 225 (153 residues), 165.2 bits, see alignment E=5.1e-53

Best Hits

Swiss-Prot: 68% identical to RLUF_SALTI: 23S rRNA pseudouridine(2604) synthase (rluF) from Salmonella typhi

KEGG orthology group: K06182, ribosomal large subunit pseudouridine synthase F [EC: 5.4.99.12] (inferred from 78% identity to maq:Maqu_3815)

MetaCyc: 68% identical to 23S rRNA pseudouridine2604 and tRNATyr pseudouridine35 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11835 [EC: 5.4.99.21]; 5.4.99.- [EC: 5.4.99.21]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PEU5 at UniProt or InterPro

Protein Sequence (312 amino acids)

>HP15_886 pseudouridine synthase family 1 protein (Marinobacter adhaerens HP15)
MTTGNSTRLNKYISESGMCSRREADRYIEQGNVYINGKRATVGDQVLPGDTVKVNGQVIE
PRAEEDLVFIALNKPVGIVSTTDSAEKHNIQRFVGHSERIFPIGRLDKDSQGLIFMTSNG
DLVNKILRAGNNHEKEYLVTVDKPVTREFVEGMANGVPILGTMTKKCKVSRESRFVFRIT
LVQGLNRQIRRMCEYFGYEVTKLERIRIMNVSLKGLAVGQWRDLSEKELAGLFDAIRDSS
SEAPPGAGKPAKKKSGTKAKPKPDRKPGSASTPGKPGGARRPGPKGKPAPGKKPASGKRP
KGGKPRQKSVKR