Protein Info for Psest_0090 in Pseudomonas stutzeri RCH2

Annotation: phosphonate metabolism protein PhnM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 TIGR02318: phosphonate metabolism protein PhnM" amino acids 6 to 381 (376 residues), 517 bits, see alignment E=1.4e-159 PF07969: Amidohydro_3" amino acids 125 to 359 (235 residues), 32.3 bits, see alignment E=8.2e-12 PF01979: Amidohydro_1" amino acids 159 to 361 (203 residues), 27.1 bits, see alignment E=2.6e-10

Best Hits

Swiss-Prot: 57% identical to PHNM_ECOLI: Alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase (phnM) from Escherichia coli (strain K12)

KEGG orthology group: K06162, PhnM protein (inferred from 78% identity to pmk:MDS_1777)

MetaCyc: 57% identical to RPnTP hydrolase (Escherichia coli K-12 substr. MG1655)
RXN-17955 [EC: 3.6.1.63]; 3.6.1.63 [EC: 3.6.1.63]

Predicted SEED Role

"Metal-dependent hydrolase involved in phosphonate metabolism" in subsystem Alkylphosphonate utilization

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GD96 at UniProt or InterPro

Protein Sequence (381 amino acids)

>Psest_0090 phosphonate metabolism protein PhnM (Pseudomonas stutzeri RCH2)
MPAEQILSNARLVLADQVIHGSLLIRDGLIADISAGASSLPQAQDLDGALLLPGLVELHT
DNLEKHMSPRPGVDWPSASAVLTHDAQIVAAGITTVFDALSIGDINPKGKRMQQLGVMLE
AIAAAEAAGQTRAEHRLHLRCEVSHPQCLELFRELVEQPLVQLVSVMDHAPGQRQFARME
KYREYYMGKYHLSPAEMDRFVSEQLANSARHSDQQRAAIVDICRSRELALASHDDATLEH
VTESADYGMAIAEFPTTFEAAEASHARGLKVLMGAPNIVRGGSHSGNIAAADLAQRGVLD
ILSSDYYPASLLQAALGLAEQDNGYDLPRAIATISLAPARAAGLDDRGEIAVGLRADLVQ
AQVHNGQPVIGQVWRQGRRVF