Protein Info for PGA1_c08760 in Phaeobacter inhibens DSM 17395

Annotation: sarcosine oxidase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 973 TIGR01372: sarcosine oxidase, alpha subunit family" amino acids 12 to 972 (961 residues), 1239.1 bits, see alignment E=0 PF13510: Fer2_4" amino acids 13 to 97 (85 residues), 88.1 bits, see alignment E=1.5e-28 PF00890: FAD_binding_2" amino acids 167 to 202 (36 residues), 26.4 bits, see alignment (E = 1.8e-09) PF12831: FAD_oxidored" amino acids 167 to 208 (42 residues), 34.9 bits, see alignment (E = 5.2e-12) PF03486: HI0933_like" amino acids 167 to 205 (39 residues), 31.1 bits, see alignment (E = 5.1e-11) PF07992: Pyr_redox_2" amino acids 167 to 287 (121 residues), 35 bits, see alignment E=5e-12 PF13450: NAD_binding_8" amino acids 170 to 237 (68 residues), 28.2 bits, see alignment 8.5e-10 PF17806: SO_alpha_A3" amino acids 491 to 575 (85 residues), 124.8 bits, see alignment E=6.3e-40 PF01571: GCV_T" amino acids 590 to 851 (262 residues), 202.1 bits, see alignment E=4.3e-63 PF08669: GCV_T_C" amino acids 879 to 965 (87 residues), 37.1 bits, see alignment E=1.1e-12

Best Hits

KEGG orthology group: K00302, sarcosine oxidase, subunit alpha [EC: 1.5.3.1] (inferred from 69% identity to sil:SPO1746)

Predicted SEED Role

"Sarcosine oxidase alpha subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EV35 at UniProt or InterPro

Protein Sequence (973 amino acids)

>PGA1_c08760 sarcosine oxidase subunit alpha (Phaeobacter inhibens DSM 17395)
MRIAGWAGRSDTREVGFSFNGRALTGREGEPLAAALLANNIHLVGRSFKYHRPRGILTAG
SEEPNALVTVGEGAAQEPNLRATTLPLYEGLAARSQNCWPSVGFDVMAVNDLAAPFLGAG
FYYKTFMWPAAFWEKIYEPVIRRAAGLGALSGAPDDGIYEKAYGFCDLLVIGAGPAGLMA
ALTAARGGADVILCDEDSQPGGRLLSEQETINGNPAQQWVAATLAELDSLNNVRRMARTS
VTGVYDQGTYGALETVPAGGGKPARACFWRLVAKRAILAAGALERPVAFANNDRPGIMLA
GAVRSYLNRWGVAPGERVTVFANNDDAHRTARDLLAAGVHVAAVIDSRHDAPQSDAFRVI
RGAQVCNASGRQRLESLTLRSVSGEEKLQTDCLAMSGGWNPTLHLTCHLNGRPRWRRDLA
CFVPQDGAVPGLEAVGACRGRFTTMAVLQDGAEAGARALEALGKRAVAAELPEAEQAEYR
ISPLWSVPGRGRAWVDFQNDVTVKDICQAGRENFRSAEHMKRYTTQGMATDQGKSSNVVA
LAVLAEATGRSIPETGTTTFRPPYVPVPIGVMGAGAEDQGFAPRRLLTSHRLAEDMGAHM
MEVGLWYRASYFPKPGETHWRQSCDREVGYVRNAVGVADVSTLGKIDIQGPDAARLLDFV
YANGFAKLKPGRVRYGLMLREDGFVMDDGTTACLAPNHYVMTTTTAAAGQVMAHLEFVTQ
ALHPDWDVRINSVTEQWAQFSVAGPKARALLQGLLGVAFEPADWPFMACGEIALGEIPAR
LFRISFSGEEAYELAVPARYGDSLFDELLRRAEGLGGGAYGLEALNVLRIEKGFLTHAEI
NGTATLHDLGFGGMLASGKPCVGRELAQREGLTAKDRMRLVGIKPIGAVQQLTAGAHLFD
PQDPVERVHDQGYVTSVGYSPTLGHMIGLALIKAGPDRIGDPIRMVDHTAGLDTLCEIVE
PVFFDPEGERARG