Protein Info for Psest_0870 in Pseudomonas stutzeri RCH2
Updated annotation (from data): Alpha-glucosidase (EC 3.2.1.20)
Rationale: Specifically important for utilizing D-Maltose monohydrate. Automated validation from mutant phenotype: the predicted function (3.2.1.20) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Glycosidases
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 97% identity to psa:PST_3479)Predicted SEED Role
"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)
MetaCyc Pathways
- glycogen degradation I (7/8 steps found)
- starch degradation I (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.20
Use Curated BLAST to search for 3.2.1.20
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GFF4 at UniProt or InterPro
Protein Sequence (542 amino acids)
>Psest_0870 Alpha-glucosidase (EC 3.2.1.20) (Pseudomonas stutzeri RCH2) MSEQLQNWWRGGVIYQVYPRSFFDSNGDGVGDLPGVLHKLDYIASLNVDAIWLSPFFTSP MKDFGYDVADYRGVDPLFGTLDDFVRLVEACHERGMRVLIDQVLNHSSDQHPWFAESRSS RDNDKADWYVWADPKPDGTVPNNWLSVFGGPAWSWDSRRRQYYLHNFLSSQPDLNFHCPA VQDQLLDDMEFWLKLGVDGFRLDAANFYFHDAELRDNPPNTEIREGSIGVRIDNPYAYQR HIYDKTRPENMDFLRRLRALLQRYPGASSVAEIGCDESLRTMAAYTSGGDTLHMAYSFDL LTEQCSPGYIRHTVEGIERELADGWSCWSMGNHDVVRVMTRWALNGRPDPERGRLLMALL LSLRGSVCMYQGEELGLPEAELRYEDLVDPYGITFWPEFKGRDGCRTPMPWESEAHHAGF TGSQPWLPVDDSHRSLSVAAQDADPHSMLNCYRRFLGWRREQRLLIEGDIHMVYHDDALL VFERRLGDEAWLCLFNLGDLSRSYELPAQAVPLVDVPASFAEYDGHWARLPAHGFGYVRL AG