Protein Info for Psest_0863 in Pseudomonas stutzeri RCH2

Updated annotation (from data): maltose ABC transporter, substrate-binding component MalE
Rationale: Specifically important for utilizing D-Maltose monohydrate.
Original annotation: Maltose-binding periplasmic proteins/domains

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 395 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01547: SBP_bac_1" amino acids 43 to 308 (266 residues), 140.8 bits, see alignment E=1e-44 PF13416: SBP_bac_8" amino acids 49 to 343 (295 residues), 135.7 bits, see alignment E=3e-43

Best Hits

Swiss-Prot: 57% identical to MALE_SALTY: Maltose/maltodextrin-binding periplasmic protein (malE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K10108, maltose/maltodextrin transport system substrate-binding protein (inferred from 95% identity to psa:PST_3486)

MetaCyc: 57% identical to maltose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-16-RXN [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]

Predicted SEED Role

"Maltose/maltodextrin ABC transporter, substrate binding periplasmic protein MalE" in subsystem Bacterial Chemotaxis or Maltose and Maltodextrin Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHI1 at UniProt or InterPro

Protein Sequence (395 amino acids)

>Psest_0863 maltose ABC transporter, substrate-binding component MalE (Pseudomonas stutzeri RCH2)
MNKKFWCIATVGLAATFSLPLPALAAIEEGKLVVWINGDKGYKGLAEVGKKFTAETGIPV
EVAHPDSATDKFQQAAATGNGPDIFIWAHDRIGEWAKSGLLTPVTPSADTKSGIADFSWQ
AVTYDNKLWGYPISVETIGLIYNKALVDTPPKTFDDVMALNEKLAAQGKRAILWDYNNTY
FTWPLLSAKGGYVFEHADGGYDVKSTGVNNAGAKAGAQVLRDLIDKGVMPKGADYSVAEA
AFNKGDSAMMISGPWAWSNIEKSGIDFGVAPIPAIDGEPGKPFVGVAAALLNAASPNKDL
AVEFLENYLLQVEGLKTVNADVPLGAVANTAYMEELSANPHIKATFENAQMGEPMPNVPE
MGAFWSSMAAALTNITSGRQDVDAALDDAAKRITR