Protein Info for PGA1_c08630 in Phaeobacter inhibens DSM 17395

Annotation: single-stranded-DNA-specific exonuclease RecJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 580 transmembrane" amino acids 199 to 215 (17 residues), see Phobius details amino acids 230 to 249 (20 residues), see Phobius details TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 38 to 576 (539 residues), 482.6 bits, see alignment E=6.2e-149 PF01368: DHH" amino acids 90 to 240 (151 residues), 70.3 bits, see alignment E=2.8e-23 PF02272: DHHA1" amino acids 361 to 453 (93 residues), 61.7 bits, see alignment E=1.2e-20 PF17768: RecJ_OB" amino acids 468 to 576 (109 residues), 77.7 bits, see alignment E=1e-25

Best Hits

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 87% identity to sit:TM1040_2166)

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EK55 at UniProt or InterPro

Protein Sequence (580 amino acids)

>PGA1_c08630 single-stranded-DNA-specific exonuclease RecJ (Phaeobacter inhibens DSM 17395)
MSFLGVETSLSGRRWIGPDVEVARAAEMMEQQTALPAAVCQVLARRGVPSHEASGFLTPQ
LKELLPDPRRMKDMEPAAARFLQAVERCERIAVFADYDVDGGSSAALLLVWLNQVGLSAT
LYVPDRIDEGYGPNDAAMAALAREHDLIVCVDCGTLSHGPIAAAKGADVIVLDHHLGGET
LPDCVAVVNPNRQDEDGDLGYFCAAGVVFLMLVELRRQMRDKGLGTGPDLMALLDLVALA
TVADVAPLIEANRALVRQGLKVMATRQRPGLVALADVSRMDSAPSTYHLGFLLGPRVNAG
GRIGKADLGARLLATSDPAEAEAIAARLDELNTERRDIENAVRAAALEQAEARGLDAPLV
WAAGEGWHPGVVGIVASRLKEAAGRPAIVIGLDGDEGKGSGRSVSGIDLGASIQRAAAEG
LLIKGGGHKMAAGLTVARDQLEPAMARLSELLAKQGAGDLGPSDLKLDGMLMPGAATVPL
IEQIEQAGPFGAGAPAPRYGFPDLQVRFAKRVGESHLKLSLSDGLSSGIDAICFGAFDTA
LGSRLLDHGGARFHFAGRLEINTWGGRQSVQLRLEDAAEA