Protein Info for Psest_0084 in Pseudomonas stutzeri RCH2

Updated annotation (from data): alpha-ketoglutarate TRAP transporter, 4TM/12TM components
Rationale: specific phenotype on a-ketoglutarate and no other transporter for this substrate is apparent in the fitness data. (Mutants in another TRAP system, Psest_4268:Psest_4270, have a much milder defect in a-ketoglutarate utilization).
Original annotation: TRAP transporter, 4TM/12TM fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 674 transmembrane" amino acids 17 to 36 (20 residues), see Phobius details amino acids 42 to 59 (18 residues), see Phobius details amino acids 71 to 88 (18 residues), see Phobius details amino acids 100 to 118 (19 residues), see Phobius details amino acids 125 to 144 (20 residues), see Phobius details amino acids 174 to 192 (19 residues), see Phobius details amino acids 271 to 291 (21 residues), see Phobius details amino acids 297 to 315 (19 residues), see Phobius details amino acids 340 to 356 (17 residues), see Phobius details amino acids 361 to 382 (22 residues), see Phobius details amino acids 391 to 427 (37 residues), see Phobius details amino acids 447 to 479 (33 residues), see Phobius details amino acids 485 to 503 (19 residues), see Phobius details amino acids 509 to 529 (21 residues), see Phobius details amino acids 533 to 556 (24 residues), see Phobius details amino acids 567 to 588 (22 residues), see Phobius details amino acids 598 to 621 (24 residues), see Phobius details amino acids 631 to 660 (30 residues), see Phobius details TIGR02123: TRAP transporter, 4TM/12TM fusion protein" amino acids 15 to 668 (654 residues), 761.7 bits, see alignment E=3.5e-233 PF06808: DctM" amino acids 111 to 376 (266 residues), 207.9 bits, see alignment E=1.2e-65 amino acids 388 to 591 (204 residues), 87.1 bits, see alignment E=5.7e-29

Best Hits

KEGG orthology group: None (inferred from 97% identity to psa:PST_4125)

Predicted SEED Role

"TRAP transporter, 4TM/12TM fusion protein, unknown substrate 1"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GG09 at UniProt or InterPro

Protein Sequence (674 amino acids)

>Psest_0084 alpha-ketoglutarate TRAP transporter, 4TM/12TM components (Pseudomonas stutzeri RCH2)
MSESQGLHASPSEWPRALFYVALLFSIYQIVTAAFHPVSSQVLRAGHVGFLLLLVFLCYP
ARGNGKPFQPVAWLLGLAGFATFFYQWYFEADLIQRSGDMTTADMVVGLTLIVLVFEAAR
RVMGIALPIICALFLAYGLLGEYLPGDLAHRGYYLDQIVNQLSFGTEGLYGTPTYVSATY
IFLFILFGSFLEQAGMIKLFTDFAMGLFGHKLGGPAKVSVVSSALMGTITGSGVANVVTT
GQFTIPLMKRFGYRPAFAGGVEATSSMGSQIMPPVMGAVAFIMAETINVPFVEIAKAALI
PALLYFGSVFWMVHLEAKRAGLKGLPKDECPSAMAAVKERWYLLIPLVVLVWLLFSGRTP
MFAGTIGLALTAIVILGSAIILKVSNFALRIAFWIALGLLCAGFFQLGIGVIFGVIAALV
AVCWFIKGGRDTLVICLHALVEGARHAVPVGIACALVGVIIGVVSLTGVASTFAGYILAV
GENNLFLSLLLTMLTCLVLGMGIPTIPNYIITSSIAAPALLDLGVPLIVSHMFVFYFGIM
ADLTPPVALACFAAAPIAKERGLKISMWAIRIAIAGFIVPFMAVYNPALMMQGGDWGATL
YMLFKAAFAVGLWGAVFTGYLQRPMALWEKVLAFAAAASMVLAMPISDEIGFALGALFLI
QHIWRARRAEPATA