Protein Info for HP15_811 in Marinobacter adhaerens HP15

Annotation: acetyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 20 to 643 (624 residues), 1029 bits, E=0
• PF16177: ACAS_N
  • amino acids 24 to 81 (58 residues), 75 bits, 4.9e-25
• PF00501: AMP-binding
  • amino acids 83 to 467 (385 residues), 279.6 bits, E=5.4e-87
• PF13193: AMP-binding_C
  • amino acids 532 to 610 (79 residues), 92 bits, E=5.7e-30

Best Hits

Swiss-Prot: 92% identical to ACSA_MARHV: Acetyl-coenzyme A synthetase (acsA) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 92% identity to maq:Maqu_2219)

MetaCyc: 71% identical to acetyl-CoA synthetase (AMP-forming) (Escherichia coli K-12 substr. MG1655)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• 2-methylcitrate cycle I (5/5 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• 2-methylcitrate cycle II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• acrylate degradation II (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• β-alanine biosynthesis II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• chitin deacetylation (2/4 steps found)
• cis-geranyl-CoA degradation (4/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Sequence Analysis Tools

See E4PR40 at UniProt or InterPro

Protein Sequence (649 amino acids)

>HP15_811 acetyl-CoA synthetase (Marinobacter adhaerens HP15)
MTGKQVYPVSPEVAKQALLNREQYEEMYRQSVEDPDTFWGEHGKRLEWIKPYTKVKNTTY
DYNNLSIKWFEDGQLNASANCLDRHLEKRGDQTAIIFEGDDPADSRNVTYRELHEETSKF
ANVLKGLGVKKGDVVTIYMPMIVETAVAMLACARIGAIHSVVFGGFSPEALGARIVNGKS
RFVVTADEGVRGGRKIPLKKNVDAALKNEDAANVEKVVVVTRTGNSEVPWNEARDERYED
LMKSASADCQPEPMNAEDPLFMLYTSGSTGAPKGVLHTTGGYMVYASMTHEYVFDYHEGD
VYWCTADFGWVTGHSYILYGPLANGAITVLFEGVPNYPDSSRMGQVVDKHKVNILYTAPT
AIRALMAEGESCMNGTTRESLKLLGSVGEPINPEAWEWYHRVIGNSKCPIVDTWWQTETG
GILISPLPGAIDLKPGSATVPFFGVQPALVDNDGNILEGKTEGNLVILDSWPGQMRTIYG
DHERFAQTYFSTYKGMYFTGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEVESALVSH
EKVAEAAVVGYPHDIKGQGIYVYVTLVHGQEPSDELKKELVQWVRKEIGPIASPDVIQWA
PGLPKTRSGKIMRRILRKIAANEHDQLGDTSTLADPGVVDDLISGRANQ