Protein Info for Psest_0832 in Pseudomonas stutzeri RCH2

Annotation: iron-sulfur cluster repair di-iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 239 PF04405: ScdA_N" amino acids 7 to 60 (54 residues), 71.3 bits, see alignment E=4e-24 TIGR03652: iron-sulfur cluster repair di-iron protein" amino acids 10 to 218 (209 residues), 258.5 bits, see alignment E=2.7e-81 PF01814: Hemerythrin" amino acids 80 to 217 (138 residues), 93.5 bits, see alignment E=1.5e-30

Best Hits

Swiss-Prot: 53% identical to YTFE_CITK8: Iron-sulfur cluster repair protein YtfE (ytfE) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: K07322, regulator of cell morphogenesis and NO signaling (inferred from 65% identity to pba:PSEBR_a3161)

MetaCyc: 52% identical to iron-sulfur cluster repair protein YtfE (Escherichia coli K-12 substr. MG1655)
1.7.99.-

Predicted SEED Role

"Nitric oxide-dependent regulator DnrN or NorA" in subsystem Nitrosative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ48 at UniProt or InterPro

Protein Sequence (239 amino acids)

>Psest_0832 iron-sulfur cluster repair di-iron protein (Pseudomonas stutzeri RCH2)
MTTALIDQTLGNLACSVAGATRVFRQYRLDFCCGGDLPLREAAQRIGVDPETIAAALLAL
EPNAEEQDWRTVPAGQLIEYILARFHERHRDQFPELIRLASRVEQVHGSRPECPNGLAEH
LWNMQQELESHMLKEEQILFPMLQRGMGFPQAQGPISVMRYEHQEHGNALERLAALTNDI
TPPANACNTWRALYRGLEEMRSDLMQHIHLENNVLFRNAEAPARIPTVQATESAEEVRP