Protein Info for Psest_0811 in Pseudomonas stutzeri RCH2

Annotation: heme d1 biosynthesis radical SAM protein NirJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 393 TIGR04051: heme d1 biosynthesis radical SAM protein NirJ" amino acids 30 to 381 (352 residues), 620.1 bits, see alignment E=1e-190 PF04055: Radical_SAM" amino acids 35 to 174 (140 residues), 94.3 bits, see alignment E=1.5e-30 PF13353: Fer4_12" amino acids 37 to 138 (102 residues), 31.8 bits, see alignment E=2.4e-11 PF13186: SPASM" amino acids 289 to 346 (58 residues), 21.7 bits, see alignment E=3.1e-08

Best Hits

KEGG orthology group: None (inferred from 92% identity to psa:PST_3536)

Predicted SEED Role

"Heme d1 biosynthesis protein NirJ" in subsystem Dissimilatory nitrite reductase or Heme biosynthesis orphans

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJA5 at UniProt or InterPro

Protein Sequence (393 amino acids)

>Psest_0811 heme d1 biosynthesis radical SAM protein NirJ (Pseudomonas stutzeri RCH2)
MLRISHYLRALAQPATQVLGARSTSGKRPPVVIWNLLRRCNLTCKHCYATSADSEFRDEL
DTDEALKVIDELHEAGVRVLILSGGEPLLRPDIFLLADYARDKGFFVALSTNGTLIDENN
IGRIAAAQFDYVGISIDGLEAVHDEWRQLKGSFAASMHAIDLCRQRDIRVGLRTTLTQNN
YPQLPALLALMREHDVQKFYLSHLNYSGRGKRSSKADAHHQMTRDAMRQLFDQAWDDVQH
GRETDFVSGNNDADAILLLQWAEERLPEHRDRLEGMLRAWGGNASGSGIANIDNIGDVHP
DTYWWQHTVGNVRRQRFSDIWLNDPAPLLQELRQHPRAVGGRCADCRWLAICNGNTRTRA
WAQGDLWAEDPGCYLTDAEIGRPAAERIPSIAI