Protein Info for Psest_0806 in Pseudomonas stutzeri RCH2

Annotation: prepilin-type N-terminal cleavage/methylation domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details PF07963: N_methyl" amino acids 14 to 35 (22 residues), 23.5 bits, see alignment (E = 3.1e-09) TIGR02532: prepilin-type N-terminal cleavage/methylation domain" amino acids 15 to 36 (22 residues), 18.7 bits, see alignment (E = 6.1e-08) PF16074: PilW" amino acids 210 to 363 (154 residues), 76.1 bits, see alignment E=2.6e-25

Best Hits

KEGG orthology group: K02672, type IV pilus assembly protein PilW (inferred from 47% identity to avn:Avin_11850)

Predicted SEED Role

"Type IV fimbrial biogenesis protein PilW" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJA1 at UniProt or InterPro

Protein Sequence (371 amino acids)

>Psest_0806 prepilin-type N-terminal cleavage/methylation domain (Pseudomonas stutzeri RCH2)
MRRPSPIRYRASAEGFGLIELMVAMTIALILSAATLTLYLNMSRSNSELAKTSSQMEHGR
LAIRLLRDDLMHAGFLNGFVPQFDDLTYAAVPSDYPADIPAPCAPYSDWNAAYKTNLIGL
PVQLYAAVPASCSGVMPSALVGSDVLLVRRVASCVTGSPNCEANVSGKLYWQTGYCDSEP
RYVLGSSGFTLHKRDCTTQAETRRFLNNAYYIRDYAVTAGDGIPTLMQSTFDLDSGALDQ
QAAVPMVEGIEAMRFVFGVDDLGDTGLPVDYEQPVQWASELNRNSPTNRGDGAPDSTCTS
ATPCELEQLTNTVVVQVHLLVRNIEPTPGYQSGKTYNLGDQSFGPFTDAFQRHVYSTTVR
LTNVSARRETP