Protein Info for HP15_766 in Marinobacter adhaerens HP15

Annotation: bacterial RecJ exonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 577 TIGR00644: single-stranded-DNA-specific exonuclease RecJ" amino acids 24 to 571 (548 residues), 484.8 bits, see alignment E=1.4e-149 PF01368: DHH" amino acids 73 to 232 (160 residues), 92.9 bits, see alignment E=2.8e-30 PF02272: DHHA1" amino acids 360 to 454 (95 residues), 68.4 bits, see alignment E=9.9e-23 PF17768: RecJ_OB" amino acids 468 to 571 (104 residues), 82.4 bits, see alignment E=3.6e-27

Best Hits

Swiss-Prot: 54% identical to RECJ_ECOLI: Single-stranded-DNA-specific exonuclease RecJ (recJ) from Escherichia coli (strain K12)

KEGG orthology group: K07462, single-stranded-DNA-specific exonuclease [EC: 3.1.-.-] (inferred from 88% identity to maq:Maqu_2272)

MetaCyc: 54% identical to ssDNA-specific exonuclease RecJ (Escherichia coli K-12 substr. MG1655)
Exodeoxyribonuclease VII. [EC: 3.1.11.6]

Predicted SEED Role

"Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-)" in subsystem DNA-replication or DNA Repair Base Excision or DNA repair, bacterial RecFOR pathway (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-, 3.1.11.6

Use Curated BLAST to search for 3.1.-.- or 3.1.11.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PQI7 at UniProt or InterPro

Protein Sequence (577 amino acids)

>HP15_766 bacterial RecJ exonuclease (Marinobacter adhaerens HP15)
MTPKKILRRPQPDTFPDWGQNLPPLLRRLYAARGVTSDEQLSYTLKHLASPLSLRGIDRA
VELLAEAIDQQQRVLVLGDFDADGATSTAVAMLGLSMLGLQSIDFRVPSRFADGYGLTPG
IIERLRDEGQLPDLMVTVDNGISAVEGVRAAKELGVKVMVTDHHLAGEELPDADAIVNPN
QPGCPFLSKNAAGVGVMFYVLTALRKQLRETNRLPDPEPNLGCLLDLVALGTVADVVPLD
HNNRIFVEQGLRRIRQGEARPGILALLEVAGRDHTAISSTDLGFVVGPRLNAAGRLDDMS
IGIACLLADSPDEARRLARELDTFNRERRTIEKDMKAQAQDLLSSMSLDLDGLPWGLALF
DTDWHQGVIGILAARIREQTHRPTIAFAPDENGVDIKGSARSIPGLHIRDVLAVVDSRHP
GIMKKFGGHAMAAGMTLARDDLDAFSEAFDRAVRDTLSAEDLEAAITTDGPLGASELSLD
TAALLKRAGPWGQHFPEPLFDGEFRVVSQRIVGENHLKLVLQPVEGGGIIDGIAFNTGPE
VPDYTRTGARVVYKPDANTFRGRTNLQLLVDYLEPLA