Protein Info for Psest_0797 in Pseudomonas stutzeri RCH2

Annotation: Predicted lipoprotein involved in nitrous oxide reduction

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 190 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF05573: NosL" amino acids 36 to 163 (128 residues), 156.6 bits, see alignment E=2.3e-50

Best Hits

Swiss-Prot: 88% identical to NOSL_PSEST: Copper-binding lipoprotein NosL (nosL) from Pseudomonas stutzeri

KEGG orthology group: None (inferred from 85% identity to psa:PST_3546)

Predicted SEED Role

"Nitrous oxide reductase maturation protein, outer-membrane lipoprotein NosL" in subsystem Denitrification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ11 at UniProt or InterPro

Protein Sequence (190 amino acids)

>Psest_0797 Predicted lipoprotein involved in nitrous oxide reduction (Pseudomonas stutzeri RCH2)
MNALHRIGAGTLFALALAFTLAGCGEKEEVQQSLEPVAFHDSDECHVCGMIITDFPGPKG
QAVEKGGVRKFCSTAEMLGWWLQPENRLLNAKLYVHDMGRSVWEHPDDGHLIDATSAYYV
VGTSLKGAMGASLATFAEEQAALRLAEEHGGRVLRFDQIDQALLQEAASMQHGGMHGHMP
SDSHNAHSGH