Protein Info for Psest_0794 in Pseudomonas stutzeri RCH2

Annotation: parallel beta-helix repeat (two copies)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR04247: nitrous oxide reductase family maturation protein NosD" amino acids 43 to 421 (379 residues), 461.9 bits, see alignment E=1.2e-142 PF05048: NosD" amino acids 144 to 357 (214 residues), 188.1 bits, see alignment E=1.4e-59 PF13229: Beta_helix" amino acids 160 to 326 (167 residues), 64.7 bits, see alignment E=7.9e-22 TIGR03804: parallel beta-helix repeat" amino acids 161 to 201 (41 residues), 23.1 bits, see alignment 4.5e-09 amino acids 289 to 330 (42 residues), 24.4 bits, see alignment 1.8e-09

Best Hits

Swiss-Prot: 94% identical to NOSD_PSEST: Probable ABC transporter binding protein NosD (nosD) from Pseudomonas stutzeri

KEGG orthology group: K07218, nitrous oxidase accessory protein (inferred from 86% identity to psa:PST_3549)

Predicted SEED Role

"Nitrous oxide reductase maturation protein NosD" in subsystem Denitrification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHX4 at UniProt or InterPro

Protein Sequence (436 amino acids)

>Psest_0794 parallel beta-helix repeat (two copies) (Pseudomonas stutzeri RCH2)
MFKAQAIFSRYSAAVSLLLLFSGVAQAAPQPITTLPLQPDGENRWRLPAGEYQGQFTIEQ
PMQLRCEPGAIIQSQRQGSSLLISAPDVLVDGCTLREWGADLTAMDSAVFILPAAERAQI
SNNRMRGPGFGVFVDGTSDVQVLGNQIDGDASVRSQDRGNGIHLFAVKGARIVGNQVRDV
RDGIYIDTSNGNHLEGNVIEDVRYGVHYMFANDNSVIDNITRRTRTGYALMQSRKLIVTG
NRSEQDQNYGILLNYITYSTIKDNFVSDVQRGDTGGDSMISGGEGKALFIYNSLFNTIEN
NHFEKSSLGIHLTAGSEDNRISGNAFVGNQQQVKYVASRTQEWSVDGRGNYWSDYLGWDR
NNDGLGDVAYEPNDNVDRLLWLYPQVRLLMNSPSIEVLRWVQRAFPVVKSPGVQDSHPLM
KLPTEKLLTEKQEPTS