Protein Info for Psest_0793 in Pseudomonas stutzeri RCH2

Annotation: Nitrous oxide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 signal peptide" amino acids 20 to 21 (2 residues), see Phobius details transmembrane" amino acids 22 to 42 (21 residues), see Phobius details TIGR04244: nitrous-oxide reductase, TAT-dependent" amino acids 16 to 635 (620 residues), 1041.3 bits, see alignment E=0 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 18 to 49 (32 residues), 20.7 bits, see alignment (E = 3.9e-08) PF18793: nos_propeller_2" amino acids 170 to 240 (71 residues), 108.1 bits, see alignment E=3.5e-35 PF18764: nos_propeller" amino acids 423 to 493 (71 residues), 117.8 bits, see alignment E=2.4e-38

Best Hits

Swiss-Prot: 91% identical to NOSZ_PSEST: Nitrous-oxide reductase (nosZ) from Pseudomonas stutzeri

KEGG orthology group: K00376, nitrous-oxide reductase [EC: 1.7.2.4] (inferred from 96% identity to psa:PST_3550)

MetaCyc: 91% identical to nitrous oxide reductase subunit (Stutzerimonas stutzeri)
RXN-12130 [EC: 1.7.2.4]

Predicted SEED Role

"Nitrous-oxide reductase (EC 1.7.99.6)" in subsystem Denitrification (EC 1.7.99.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.2.4 or 1.7.99.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHA6 at UniProt or InterPro

Protein Sequence (637 amino acids)

>Psest_0793 Nitrous oxide reductase (Pseudomonas stutzeri RCH2)
MSDKSKNNPEVLEKGGMSRRGFLGASAVTGAAVAATAFGGAVMSRESWAAAVKNAQQNIH
VAPGELDDYYGFWSGGHQGEVRVMGIPSMRELMRIPVFNVDSATGWGLTNESRAIMGDSA
KFLNGDCHHPHISMTDGKYDGKYLFINDKANTRVARIRLDIMKCDKMLTVPNCQAIHGLR
LQKVPYTKYVFANAEFVIPHPNDGKVFDLQDENSYTMYNAIDAETMEMAFQIIVDGNLDN
TDMDYTGRFTAATCYNSEKAYDLGGMMRNERDWVVVFDIEAAEKAVKAGKFTTLGDSKVP
VLDGRKKGDKDSEFTRYIPVPKNPHGCNTSSDGKYFIANGKLSPTVSMIEIAKLPDLFAG
KLKDPRDTIAAEVELGLGPLHTTFDGRGNAYTTLFIDSQVVKWNMADAVRAYNGEKVNYI
KQKLDVHYQPGHIHASLCETSEADGKWLVALCKFGKDRFLPTGPLHPENDQLIDISGDEM
KLVHDGPTYAEPHDCIMARRDQIKTKKIWDRNDPFFAPTLERAKKDGINLDADNKVIRDG
NKVRVYMTSMAPAFGVQEFTVKQGDEVTVTITNIDQIEDVSHGFVMVNHGVSMEISPQQT
SSITFVADKPGLHWYYCSWFCHALHMEMVGRMLVEPA