Protein Info for Psest_0791 in Pseudomonas stutzeri RCH2

Annotation: Acyl-CoA dehydrogenases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 PF02771: Acyl-CoA_dh_N" amino acids 9 to 129 (121 residues), 48.7 bits, see alignment E=1.8e-16 PF02770: Acyl-CoA_dh_M" amino acids 133 to 215 (83 residues), 69.9 bits, see alignment E=3.4e-23 PF00441: Acyl-CoA_dh_1" amino acids 245 to 393 (149 residues), 127.3 bits, see alignment E=1.2e-40 PF08028: Acyl-CoA_dh_2" amino acids 262 to 367 (106 residues), 49.4 bits, see alignment E=1.2e-16

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 96% identity to psa:PST_3552)

Predicted SEED Role

"Isovaleryl-CoA dehydrogenase (EC 1.3.8.4)" (EC 1.3.8.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.4, 1.3.99.3

Use Curated BLAST to search for 1.3.8.4 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GJ87 at UniProt or InterPro

Protein Sequence (416 amino acids)

>Psest_0791 Acyl-CoA dehydrogenases (Pseudomonas stutzeri RCH2)
MDFAYSPRVEALRQQLRAFMDQHIVPRIGAWHAEVAAGQYPVSFMDDLKALARSEGLWNL
FLPSLREDEPGMGLSNLEYAPLAEIMGRVHWASEVFNCNAPDTGNMELLHMFATPEQRQR
WLGPLLEGEIRSAFAMTEPDVPSSDATNIQTLIRRDGDDYVINGRKWFITNASHPNCKLL
IVMGKTDPDAETHQQQSMILVPFDTPGVELVRNIPVMNHIAPEGHSELLLRNVRVPASNL
LGKEGDGFMMAQARLGPGRIHHCMRSIGMAELALELMVERCQERKAFGKYLQQYSNVADW
IAESRIEIEQARLLVLKTAWMIDEVGAKAARKEISMIKALVPRMHINVVDRAIQVYGAMG
LTPDTPLADMWTGGRALRFADGPDQVHLRSIARMEIKASEATRGATAAYLTPPGRH