Protein Info for Psest_0773 in Pseudomonas stutzeri RCH2

Annotation: Cytosine deaminase and related metal-dependent hydrolases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 411 PF07969: Amidohydro_3" amino acids 41 to 384 (344 residues), 171.2 bits, see alignment E=5.9e-54 PF01979: Amidohydro_1" amino acids 48 to 376 (329 residues), 50.5 bits, see alignment E=1.9e-17

Best Hits

Swiss-Prot: 58% identical to CODA_ECOLI: Cytosine deaminase (codA) from Escherichia coli (strain K12)

KEGG orthology group: K01485, cytosine deaminase [EC: 3.5.4.1] (inferred from 98% identity to psa:PST_3570)

MetaCyc: 71% identical to cytosine deaminase monomer (Klebsiella pneumoniae)
Cytosine deaminase. [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]; 3.5.4.1 [EC: 3.5.4.1]

Predicted SEED Role

"Cytosine deaminase (EC 3.5.4.1)" (EC 3.5.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH84 at UniProt or InterPro

Protein Sequence (411 amino acids)

>Psest_0773 Cytosine deaminase and related metal-dependent hydrolases (Pseudomonas stutzeri RCH2)
MNILNARLRGRSGLYRIELDGARIASISAQQAPAEANEGDIDAASNLVVPPFIEPHIHLD
ATLTAGQPAWNMSGTLFEGIERWGERKALVTHEDTKARAKKTIDMLVDHGIQHVRTHVDV
TDPTLSALKSMLEVREETRHLIDLQIVAFPQEGIESFKGGRELMTEAIAMGADVVGGIPH
FENTRDQGVSSIKFLMDLAERTGCLVDVHCDETDDPQSRFLEVLAEEARVREIGERVTAS
HTTAMGSYDNAYCSKLFRLLKLSKINFVSCPTESIHLQGRFDTYPKRRGLTRVAEIDRAG
MNVCFGQDSIVDPWYPLGNGNILRILEAGLHICHMLGYEDLQRGLDLITDNSARTLNLGE
RYGLEVGRPANLLVLSAPDDYEMLRTQGHALLSVRNGEVLMRRTPAQIQRY