Protein Info for PGA1_c07640 in Phaeobacter inhibens DSM 17395

Annotation: O-acetylhomoserine / O-acetylserine sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 9 to 425 (417 residues), 583.7 bits, E=9.6e-180
• PF01053: Cys_Met_Meta_PP
  • amino acids 10 to 425 (416 residues), 409.7 bits, E=9.3e-127
• PF01041: DegT_DnrJ_EryC1
  • amino acids 64 to 189 (126 residues), 27.6 bits, E=1.8e-10

Best Hits

Swiss-Prot: 51% identical to CYSD_SCHPO: Homocysteine synthase (met17) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 89% identity to sil:SPO1431)

MetaCyc: 47% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• L-methionine biosynthesis II (4/6 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (3/5 steps found)
• homocysteine and cysteine interconversion (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See I7EJX0 at UniProt or InterPro

Protein Sequence (430 amino acids)

>PGA1_c07640 O-acetylhomoserine / O-acetylserine sulfhydrylase (Phaeobacter inhibens DSM 17395)
MSEAPSYGFDTLQIHAGAKPDPATGARQTPIYQTTAYVFRDADHAAALFNLQEVGFIYSR
LTNPTVAVLQERIATLEGGVGAVCCSSGHAAQIMALFPLMGPGCNVVASTRLYGGTVTQF
SQTIKRFGWSAKFVDFDNPEAVAAAIDDDTRAVFGESVANPGGYVTDIRSIADVADAAGV
PLIIDNTSATPYLCSPIAHGATLVVHSTTKYLTGNGTVTGGVIVDSGKFDWSANDKFPSL
SQPEPAYHGLKFHETFGGLAFTFHGIAIGLRDLGMTMNPQAAHYTLMGVETLSLRMERHC
ENAKTVASWLEQDPRVDYVTYAGLPSSPYHARAKEHYPKGTGGLFTFAVKGGYDACVKLV
NSLEIFSHVANLGDTRSLIIHSASTTHRQLTPEQQEAAGAGANVVRVSIGIENADDLIAD
LDQALSKASS