Protein Info for HP15_725 in Marinobacter adhaerens HP15

Annotation: two component, sigma54 specific, transcriptional regulator, Fis family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 PF00072: Response_reg" amino acids 6 to 115 (110 residues), 100.4 bits, see alignment E=3.2e-32 PF14532: Sigma54_activ_2" amino acids 146 to 316 (171 residues), 63.8 bits, see alignment E=9.8e-21 PF00158: Sigma54_activat" amino acids 146 to 311 (166 residues), 229.6 bits, see alignment E=8.1e-72 PF07724: AAA_2" amino acids 167 to 297 (131 residues), 32 bits, see alignment E=5.7e-11 PF07728: AAA_5" amino acids 168 to 286 (119 residues), 26.5 bits, see alignment E=2.6e-09 PF25601: AAA_lid_14" amino acids 317 to 376 (60 residues), 63 bits, see alignment E=8.4e-21 PF02954: HTH_8" amino acids 404 to 441 (38 residues), 28.4 bits, see alignment 5.1e-10

Best Hits

Swiss-Prot: 52% identical to DCTD_RHIME: C4-dicarboxylate transport transcriptional regulatory protein DctD (dctD) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K10126, two-component system, NtrC family, C4-dicarboxylate transport response regulator DctD (inferred from 85% identity to maq:Maqu_2347)

Predicted SEED Role

"Response regulator of zinc sigma-54-dependent two-component system" in subsystem Zinc resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PQE6 at UniProt or InterPro

Protein Sequence (450 amino acids)

>HP15_725 two component, sigma54 specific, transcriptional regulator, Fis family (Marinobacter adhaerens HP15)
MTEPSVIFVDDDPHIRQAIAQTLTLEELPVSCFEDAPSALGHINADYDGVVLCDYNMPEM
NGLEMLDRLRAIDDTIPVIILTGQGDISTAVTAMQQGAYDFIEKPFDHDELIELLRHALE
KRHLALENRRLKAQLRHLARPGPRMLGDSPLMQKVMATIDPILDISANILLHGETGSGKD
ALARYIHENSGRSAHNFVAINCGAVPENLIESELFGHEAGAFTGAEKRRIGKIEHAHKGT
LFLDEVESMPMPLQVKLLRVLEEQRVERLGSNKVQEVDVRIIAATKADLKKLSDDGEFRP
DLYYRLNVVKVDIPPLRERKEDIPLLFHHFVLIAAARYDRESIPLDAGQAARLMQHSWPG
NVRELRNLAERYVLLGPAALDENEPADTANVAGRQTLAEMMDGFERSAIISALNASHGSI
KDTMVQLGIARKTLYDKMRKHGLDKAQFKD