Protein Info for Psest_0738 in Pseudomonas stutzeri RCH2

Annotation: copper-(or silver)-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 792 transmembrane" amino acids 155 to 173 (19 residues), see Phobius details amino acids 184 to 204 (21 residues), see Phobius details amino acids 217 to 237 (21 residues), see Phobius details amino acids 245 to 265 (21 residues), see Phobius details amino acids 402 to 421 (20 residues), see Phobius details amino acids 428 to 453 (26 residues), see Phobius details amino acids 743 to 761 (19 residues), see Phobius details amino acids 767 to 785 (19 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 6 to 67 (62 residues), 33.7 bits, see alignment 8e-12 PF00403: HMA" amino acids 7 to 65 (59 residues), 64.6 bits, see alignment 1.3e-21 amino acids 74 to 133 (60 residues), 59.9 bits, see alignment 3.9e-20 TIGR01511: copper-translocating P-type ATPase" amino acids 199 to 786 (588 residues), 585.5 bits, see alignment E=3.2e-179 TIGR01525: heavy metal translocating P-type ATPase" amino acids 217 to 785 (569 residues), 629.8 bits, see alignment E=1.3e-192 TIGR01494: HAD ATPase, P-type, family IC" amino acids 255 to 767 (513 residues), 282.6 bits, see alignment E=1.2e-87 PF00122: E1-E2_ATPase" amino acids 283 to 462 (180 residues), 185.7 bits, see alignment E=8.8e-59 PF00702: Hydrolase" amino acids 479 to 697 (219 residues), 107.5 bits, see alignment E=1.8e-34

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 96% identity to psa:PST_3613)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GH46 at UniProt or InterPro

Protein Sequence (792 amino acids)

>Psest_0738 copper-(or silver)-translocating P-type ATPase (Pseudomonas stutzeri RCH2)
MSSATHTLPVSGMTCASCAGRVERALLKVPGVAAANVNLANEQVRIEGDDLGVATLIEAV
EKAGYGVPLQSIELNIEGMTCASCVGRVERALLKVPGVRSAAVNLASERAHVEVIGTPDP
AVLIQAVEAAGYKASAGDQQHPEEDAERRLQRERWAVIAALLLAAPLVLPMFGELFGQHW
MLPAWIQFLLATPVQFILGARFYVAGWKAVRAGAGNMDLLVAIGTSAGYGLSLYQWWATP
AGQMPHLYFEASAVVIALVLLGKYLESRAKRQTSAAIRALEALRPDRATRVVDGREEDVA
IAALRLDDLVLVKPGERFPVDGEVVEGESQADEALISGESLPVNKAPGDRITGGAINGEG
RLLVRTTALGGETVLARIIRLVEDAQAAKAPIQKLVDKVSQVFVPAVLVIAVFTLIGWLL
TGAPVEVALINAVAVLVIACPCALGLATPAAIMAGTGVAARHGILIKDAEALEVAHAVTA
VAFDKTGTLTSGKPQIIHLHAVDGDEARILRLAGALQRGSEHPLARAVLERCEADGIAVP
DVQKSQALSGRGIAGTLDGQQLALGNRRMLEEYGLQPGELLETAQRWEAEGRTLSWLVEH
APEPRILGLFAFGDSLKDGAAAAIAGLAARHIRSHLITGDNRGSARVVAEALHIDDVHAE
VLPADKAATVAELKKGGAVVAMVGDGINDAPALAAADVGIAMGGGTDVAMHAAGITLMRG
DPRLVPAALEISRRTYRKIQQNLFWAFIYNLVGIPLAAFGFLSPVVAGAAMALSSVSVVS
NALLLRSWKPEE