Protein Info for PGA1_c00070 in Phaeobacter inhibens DSM 17395

Annotation: DNA gyrase subunit GyrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 TIGR01059: DNA gyrase, B subunit" amino acids 11 to 804 (794 residues), 995.5 bits, see alignment E=5.4e-304 PF02518: HATPase_c" amino acids 38 to 151 (114 residues), 59 bits, see alignment E=1.5e-19 PF00204: DNA_gyraseB" amino acids 229 to 398 (170 residues), 204.6 bits, see alignment E=2e-64 PF01751: Toprim" amino acids 427 to 539 (113 residues), 57.8 bits, see alignment E=2.5e-19 PF21249: GyrB_hook" amino acids 541 to 565 (25 residues), 27.8 bits, see alignment (E = 3.9e-10) PF00986: DNA_gyraseB_C" amino acids 733 to 794 (62 residues), 101.2 bits, see alignment 6.8e-33

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 92% identity to sit:TM1040_0007)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DKY7 at UniProt or InterPro

Protein Sequence (805 amino acids)

>PGA1_c00070 DNA gyrase subunit GyrB (Phaeobacter inhibens DSM 17395)
MSGNEQAPAEYGADSIKVLKGLEAVRKRPGMYIGDTDDGSGLHHMVYEVVDNGIDEALAG
HADRVVVKIHADSSVSVNDNGRGIPVGIHEEEGVSAAEVIMTQLHAGGKFDSNSYKVSGG
LHGVGVSVVNALSDWLELRIWRDGKEHVARFERGDTAEHLKVVGDCGDQTGTEVRFLAST
DTFSNLEYLFETLEKRLRELAFLNSGVRIILIDERPAERLEVELFYEGGVKEFVKYLDRS
KSPVMEAPIYITGDRDEIGVEVAMWWNDSYHETVLPFTNNIPQRDGGTHVAGFRGALTRT
INNYAQSSGIAKKEKVSFTGDDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEVRPVVESL
VNEKLAEWFEENPNQAKQIVGKIIEAALAREAARKARELTRRKTAMDVNYLAGKLKDCSE
KDPSKTEVFLVEGDSAGGSAQTGRDRMTQAILPLRGKILNVERARFDRMLGSQEIGNLVM
ALGTGIGRDEFNIDKLRYHKIVIMTDADVDGAHIRTLLLTFFYRQMPELIEGGYLYIAQP
PLYKVSRGKSEVYLKDQAALDEYLINQGVDGAVLKLGSGEELVGQDLARVVDEARQLKRV
LDAFPTHYPRHILEQAAVAGAFVPGAVDADLQGVADKVAQRLDLIALEYERGWRGRITQD
HGIRLSRILRGVEELRTLDGPMLRSGEARKTGSFTNSLQETYGATATLVRRDRSQAIHGP
LGLLKAILEEGEKGLALQRYKGLGEMNPDQLWETTLDPDARTLLQVRVEDMVEADDLFTK
LMGDVVEPRREFIQKNALSVENLDF