Protein Info for Psest_0706 in Pseudomonas stutzeri RCH2

Annotation: His Kinase A (phosphoacceptor) domain./Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase.

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 530 transmembrane" amino acids 21 to 39 (19 residues), see Phobius details amino acids 51 to 70 (20 residues), see Phobius details amino acids 77 to 96 (20 residues), see Phobius details amino acids 102 to 119 (18 residues), see Phobius details amino acids 125 to 144 (20 residues), see Phobius details amino acids 153 to 173 (21 residues), see Phobius details PF13188: PAS_8" amino acids 202 to 245 (44 residues), 22.6 bits, see alignment 1.1e-08 PF00512: HisKA" amino acids 310 to 374 (65 residues), 56.3 bits, see alignment E=4.1e-19 PF02518: HATPase_c" amino acids 417 to 523 (107 residues), 78.4 bits, see alignment E=8.5e-26

Best Hits

Swiss-Prot: 67% identical to PILS_PSEAE: Sensor protein PilS (pilS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02668, two-component system, NtrC family, sensor histidine kinase PilS [EC: 2.7.13.3] (inferred from 94% identity to psa:PST_3645)

Predicted SEED Role

"Two-component sensor PilS" in subsystem Type IV pilus

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHP4 at UniProt or InterPro

Protein Sequence (530 amino acids)

>Psest_0706 His Kinase A (phosphoacceptor) domain./Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase. (Pseudomonas stutzeri RCH2)
MGAENLTFFGDQGRRILRLYHLYRLTIGLALVLLISADLHNDLLQMSNPQLFQYGAWLYL
ILNMLVAVLVQSPERDLSVLGLALMDVILLSGLFYFGGGTPSGIGNLLIVAVAIANILLR
GRVGFFIAAVAATGLIYLTFYLSVSRPEASSQYVQAGALGTLCFAAALLVQGLTRRLQVS
ETLARQRAEEVANLEALNALILQRMRTGIMVLDPQERVLLANQGALQLLGQDSLAGERLA
PRCPDLLKALQQWRDNPTLRPRNFKAIAGGPTLQPSFAALQHGNKLDTLVFLEDTSQIAQ
KAQQLKLASLGRLTASIAHEIRNPLGAISHAAQLLHESEELDGPDRRLAQIIQDHSRRMN
LVIENVLQLSRRRQAEPQLLDLKYWLHRFVSEYRQGLNAQQTVHLETIGSSLQTRMDPNQ
LTQVLTNLVQNGLRYSGQQHGRAQVWLRLYREEASDLPVVEVLDDGPGIATEQLHNVFEP
FFTTESKGTGLGLYLSRELCESNQARLDYRERPEGGSCFRIVFAHPRKLS