Protein Info for Psest_0701 in Pseudomonas stutzeri RCH2

Annotation: uncharacterized radical SAM protein YgiQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 759 TIGR03904: uncharacterized radical SAM protein YgiQ" amino acids 23 to 642 (620 residues), 882.7 bits, see alignment E=5e-270 PF08497: Radical_SAM_N" amino acids 23 to 378 (356 residues), 462 bits, see alignment E=1.3e-142 PF04055: Radical_SAM" amino acids 379 to 583 (205 residues), 53.2 bits, see alignment E=6.5e-18 PF11842: DUF3362" amino acids 592 to 745 (154 residues), 169.7 bits, see alignment E=8.1e-54

Best Hits

Swiss-Prot: 85% identical to Y4872_PSEPK: UPF0313 protein PP_4872 (PP_4872) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 98% identity to psa:PST_3650)

Predicted SEED Role

"Fe-S OXIDOREDUCTASE (1.8.-.-)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GHP0 at UniProt or InterPro

Protein Sequence (759 amino acids)

>Psest_0701 uncharacterized radical SAM protein YgiQ (Pseudomonas stutzeri RCH2)
MHAVKPLFDYPKYWAECFGPAPFLPMSREEMDQLGWDSCDIIIVTGDAYVDHPSFGMAII
GRLLEAQGFRVGIISQPDWHSKDDFMKLGEPNLFFGVAAGNMDSMINRYTADRKVRSDDA
YTAGGLAGKRPDRASLVYSQRCKEAYKHVPVVLGGIEASLRRIAHYDYWSDKVRRSILMD
ATADILLYGNAERAIVEVAQRLARGEPVEAITDVRGTAFIRKDAPEGWFEIDSTRIDRPG
KIDKIINPYVNTQDLSACAIEKDKGPQDDPNEARPVELLPHPKLERDRTVIRLPSFEKVR
NDPALYAHANRVLHLETNPGNARALVQRHDDRELWLNPPPIPLTTEEMDYVFAAPYARVP
HPAYNGAKIPAYEMIRFSVNIMRGCFGGCTFCSITEHEGRIIQSRSHESILHEIEEMKNV
PGFTGVVSDLGGPTANMYRLACKSHEIEKHCRKPSCVFPGICENLNTDHSSLIELYRKAR
ALPGVKKILIASGLRYDLAVESPEYVKELVTHHVGGYLKIAPEHTERGPLDKMMKPGIGS
YDRFKRMFEKYSKEAGKEQYLIPYFIAAHPGTTDEDMMNLALWLKANGFRADQVQAFYPS
PMASATAMYHTGKNPLRKVTYKSEGVTVVKSEEQRRLHKAFLRYHDPKGWPLLREALIRM
GRADLIGNGKHQLIPAHQPATEGYQSARRKNSTPVGSKKTGQGGKILTQHNGLPPRSHDG
NAWDKREQAKAAAEARRKAEKSGKPAGKGGKPQRPVAPR