Protein Info for Psest_0685 in Pseudomonas stutzeri RCH2

Annotation: HflK protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 transmembrane" amino acids 71 to 93 (23 residues), see Phobius details PF12221: HflK_N" amino acids 1 to 54 (54 residues), 65.8 bits, see alignment 2.5e-22 TIGR01933: HflK protein" amino acids 90 to 350 (261 residues), 376.6 bits, see alignment E=3.2e-117 PF01145: Band_7" amino acids 92 to 263 (172 residues), 103.8 bits, see alignment E=1.1e-33

Best Hits

Swiss-Prot: 47% identical to HFLK_VIBPA: Protein HflK (hflK) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K04088, membrane protease subunit HflK [EC: 3.4.-.-] (inferred from 98% identity to psa:PST_3666)

MetaCyc: 46% identical to regulator of FtsH protease (Escherichia coli K-12 substr. MG1655)
3.4.24.-

Predicted SEED Role

"HflK protein" in subsystem Hfl operon

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-

Use Curated BLAST to search for 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GEW6 at UniProt or InterPro

Protein Sequence (392 amino acids)

>Psest_0685 HflK protein (Pseudomonas stutzeri RCH2)
MAWNEPGGNSNNQDPWGSGGGGRRGGGGDQKGPPDLDEAFRKLQDSLNGMFGGKKRGGGS
NFGGSGKRGGFGLVWVALVVLLAVWLFNAIYIVDEQEQAVVLRFGKYHETVGPGLNIYFP
PIDRKFQENVTRERSYSKQGQMLTEDENIIEVPLTVQYKISNLQSFVLSVDQPEISLQHA
TDSAVRHVVGSTAMDQVLTEGREVMAGEVKERLQRFLDNYGTGIVVTQVNLQSAAAPREV
QEAFDDVIRAREDEQREKNQAESYANGVIPEARGQAQRMLEEASGYRDAVISRATGEADR
FSKLVAEYRKAPEVTRERLYLETMQEVMSNTSKVMVSGDGGQNLLYLPLDKMINSRGAST
PAPAGVASGAATQGTRLPPELDPREVRTRESR