Protein Info for Psest_0682 in Pseudomonas stutzeri RCH2

Annotation: tRNA dimethylallyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00174: tRNA dimethylallyltransferase
  • amino acids 7 to 304 (298 residues), 337.3 bits, E=3.7e-105
• PF01715: IPPT
  • amino acids 41 to 294 (254 residues), 297.3 bits, E=1e-92

Best Hits

Swiss-Prot: 94% identical to MIAA_PSEU5: tRNA dimethylallyltransferase (miaA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 94% identity to psa:PST_3669)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

• cis-zeatin biosynthesis (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Zeatin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

See L0GIP2 at UniProt or InterPro

Protein Sequence (323 amino acids)

>Psest_0682 tRNA dimethylallyltransferase (Pseudomonas stutzeri RCH2)
MSSLPPAIFLMGPTASGKTDLALELARVLPCELISVDSALIYRGMDIGTAKPSAEVLAEF
PHRLVDILDPAESYSAAEFSADALAAMAEITALGRIPLLVGGTMLYFKALQDGLADMPAA
DPLVRAQLEALAASEGLLALHRELAQVDPESAARIHPNDPQRLVRALEVYLVSGLTMSEH
RARQRSQKAAADAPGSGVLPYTVAQLCIAPAQRHILHERIESRFRHMVEQGFVEEVEALR
RRGDLHPGMPSIRAVGYRQVWEYLDGSSTREEMVERGIIATRQLAKRQFTWLRSWGEIHW
LDSLSRDNLPRALKYLQSLSILS