Protein Info for Psest_0681 in Pseudomonas stutzeri RCH2

Annotation: DNA mismatch repair protein MutL

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 625 TIGR00585: DNA mismatch repair protein MutL" amino acids 6 to 314 (309 residues), 341.9 bits, see alignment E=2.5e-106 PF02518: HATPase_c" amino acids 25 to 81 (57 residues), 36.5 bits, see alignment 1.2e-12 PF13589: HATPase_c_3" amino acids 28 to 130 (103 residues), 44.5 bits, see alignment E=3.4e-15 PF01119: DNA_mis_repair" amino acids 217 to 333 (117 residues), 143.7 bits, see alignment E=4e-46 PF08676: MutL_C" amino acids 439 to 581 (143 residues), 169.1 bits, see alignment E=9.3e-54

Best Hits

Swiss-Prot: 96% identical to MUTL_PSEU5: DNA mismatch repair protein MutL (mutL) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03572, DNA mismatch repair protein MutL (inferred from 96% identity to psa:PST_3670)

Predicted SEED Role

"DNA mismatch repair protein MutL" in subsystem DNA repair, bacterial MutL-MutS system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIY7 at UniProt or InterPro

Protein Sequence (625 amino acids)

>Psest_0681 DNA mismatch repair protein MutL (Pseudomonas stutzeri RCH2)
MTDTPRIQLLSPRLANQIAAGEVVERPASVIKELLENSLDSGASRIEVDVEQGGVKLLRV
RDDGCGIPPDDLPLALARHATSKIRDLEDLERVMSLGFRGEALASISSVSRLTMTSRTAD
ASEAWQVETEGREMEARVQPAAHPVGTSVEVRDLFFNTPARRKFLRTEKTEFDHLQEVIK
RLALARFDVAFHLRHNGKAVLALHQASDDASRARRVAAVCGSAFLEQALPIEIERNGLRL
WGWVGLPTFSRSQADLQYFYVNGRMVRDKLVAHAVRQAYRDVLFNGRHPTFVLFLDVDPA
VVDVNVHPTKHEVRFRDSRMVHDFLYGTLHRALGDVRPEDQLAAPASVTSMTQVSGLAAG
EFGGQNEMSLAASVLERPPGETPAWRGAGAGYQAPRPAAAGYTAETQGAYRELFTPLPES
GPAALPASQDDVPPLGYALAQLKGVYILAENAQGMVLVDMHAAHERITYERLKTAMASEG
LRGQPLLVPESIALSQREADCAEEHGQWFQMLGFELQRLGPETLGIRQIPSLLKQAEATQ
LVRDVLADLLEYGTSDRIQAHLNELLATMACHGAVRANRRLTLPEMNALLRDMEHTERSG
QCNHGRPTWTQLGMDELDKLFLRGR