Protein Info for Psest_0680 in Pseudomonas stutzeri RCH2

Annotation: N-acetylmuramoyl-L-alanine amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF11741: AMIN
  • amino acids 35 to 134 (100 residues), 83.1 bits, E=1.9e-27
• PF01520: Amidase_3
  • amino acids 181 to 399 (219 residues), 156.9 bits, E=8.1e-50
• PF01476: LysM
  • amino acids 428 to 470 (43 residues), 47.9 bits, 1.5e-16

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 96% identity to psa:PST_3671)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling II (7/10 steps found)
• peptidoglycan recycling I (9/14 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

See L0GEW0 at UniProt or InterPro

Protein Sequence (478 amino acids)

>Psest_0680 N-acetylmuramoyl-L-alanine amidase (Pseudomonas stutzeri RCH2)
MNNMGLGMRIRTFIGGLALLLMATDLLAASDVQSVRLWRAPDNTRLVFDLSGPVEHKIFT
LTAPDRLVIDVTGATLKAELDKLALQNTPVASLRAGQHDANTLRVVVDLHAPVSPKSFSL
APNQQYGHRLVVDLFDQATAARAATQPPVTATPATPAAPVSPTLPAVKLPATGGSKRDIV
IAIDAGHGGEDPGAIGPGKVYEKHVVLQISKELQRQINADKGFRAELVRTGDYFIPLRKR
TEIARKKGADLFVSIHADAAPRSAAYGASVFALSDRGATSETARWLADSENRSDLIGGAG
NVSLGDKDQMLAGVLLDLSMTASLSSSLNVGQKVLSNMGRITPLHKRRVEQAGFMVLKSP
DIPSILVETGFISNPSEAKKLQTASHQQSLARSIHSGVRQFFHENPPPGTYVAWLRDSGK
IAAGPREHIVRSGESLALLAQRYQVSLAALRSANSLSNDVIKVGQTLNIPATTLASQP