Protein Info for HP15_618 in Marinobacter adhaerens HP15
Annotation: acyl-CoA dehydrogenase domain protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 55% identical to ACADL_PIG: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) from Sus scrofa
KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 91% identity to maq:Maqu_2399)MetaCyc: 52% identical to long-chain-acyl-CoA dehydrogenase monomer (Homo sapiens)
LONG-CHAIN-ACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-21775 [EC: 1.3.8.8, 1.3.8.7]
Predicted SEED Role
"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)
MetaCyc Pathways
- oleate β-oxidation (30/35 steps found)
- fatty acid salvage (6/6 steps found)
- adipate degradation (5/5 steps found)
- pyruvate fermentation to hexanol (engineered) (8/11 steps found)
- 2-methyl-branched fatty acid β-oxidation (10/14 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
- (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Fatty acid metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2, 1.3.99.3
Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2 or 1.3.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See E4PP70 at UniProt or InterPro
Protein Sequence (393 amino acids)
>HP15_618 acyl-CoA dehydrogenase domain protein (Marinobacter adhaerens HP15) MNDNHNTGYSPRFTMIPRTLFDADLEGFRDSVRKFLEQEATPYHDQWEKDGQVSRELWQK AGELGFLCPCLPEEFGGVGADFRYSAVLIEEVARAGLTGIGWGLHSDIVAPYVLNYGSDE LKNHYLPKLASGEMIGAIAMTEPGAGSDLQGVKTTAVKNGDHYVLNGSKTFITNGQLADV VIVVAKTDPKEGAKGISLFMVETAWEGFEKGQNLNKVGMKAQDTSELFFQDVKVPEKNLI GPGEGQGFFQLMQELAAERLQVALGAVAAAEAAWQWTLDYVKERNAFGKPVIAFQNTRFK MAEMKAEITAARVFTDRCLELHLDKKLDIPTAAMLKQLTTDLQCKVMDECVQLHGGYGYM WEYPIARAWADSRVQRIYAGTNEIMKEIVARSF