Protein Info for Psest_0615 in Pseudomonas stutzeri RCH2

Annotation: RND family efflux transporter, MFP subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 16 to 35 (20 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 122 to 425 (304 residues), 161.9 bits, see alignment E=9.5e-52 PF16576: HlyD_D23" amino acids 131 to 363 (233 residues), 141.3 bits, see alignment E=5.6e-45 PF13533: Biotin_lipoyl_2" amino acids 149 to 185 (37 residues), 29.7 bits, see alignment 8.6e-11 PF13437: HlyD_3" amino acids 261 to 360 (100 residues), 62.3 bits, see alignment E=1.4e-20

Best Hits

KEGG orthology group: None (inferred from 55% identity to mms:mma_1793)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGS8 at UniProt or InterPro

Protein Sequence (448 amino acids)

>Psest_0615 RND family efflux transporter, MFP subunit (Pseudomonas stutzeri RCH2)
MKSKSNTSSLAGHKKQIFWIVVILSVALMLGGLLLRSSPAVPDAGDAHSEHGDESEHEDE
GHSDEDAEAEGDHGHDEGGADSDHEVGAAEKDEHEDEPEGAEHTETAEVELSETQILAAG
ISLATAQPAKIKSAIELPGEITFNQDRTAQVVPRLSGVVEAVKVDLGEQVKQGQVLAVIA
STDLSERRSEFYAAQKRLALAQKTYRREKELWEERISAEQDYLQAQQALREAELTVANAN
AQLQALGSDAGKPDALSRYELRAPFDGMIVEKDITLGESVNTDDQIFIISDLSTVWADIS
VPANALSAVRVGSNAVIEATAFESSANGTVSYVGSLVGQQSRAATARVTLPNPEGVWRPG
LFVKVQVGAGEASVPVAVSPDAIHTLEEKPVVFVRTDHGFAAQPIVSGRSDSDAVEIKEG
LQPGARYALDNSFIIKSELGKASASHAH