Protein Info for HP15_565 in Marinobacter adhaerens HP15

Annotation: UDP-N-acetylmuramate-L-alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 21 to 119 (99 residues), 101.7 bits, E=3.5e-33
• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 21 to 472 (452 residues), 575.9 bits, E=3.5e-177
• PF08245: Mur_ligase_M
  • amino acids 124 to 306 (183 residues), 106.5 bits, E=2.7e-34
• PF02875: Mur_ligase_C
  • amino acids 326 to 413 (88 residues), 54 bits, E=2.7e-18

Best Hits

Swiss-Prot: 90% identical to MURC_MARHV: UDP-N-acetylmuramate--L-alanine ligase (murC) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 90% identity to maq:Maqu_2451)

MetaCyc: 60% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See E4PP17 at UniProt or InterPro

Protein Sequence (481 amino acids)

>HP15_565 UDP-N-acetylmuramate-L-alanine ligase (Marinobacter adhaerens HP15)
MADANNPPLVYQVPEMRRIRHIHFVGIGGAGMSGIAEVLKNQGYDVSGSDLKEGAVTDRL
KGMGVEVQIGHREENSASADVVVVSSAVSAENPEVVAARSRRVPIVPRAEMLAEIMRYRH
GIAVAGTHGKTTTTSLIASILGEAGLDPTFVIGGKLNSAGTNAQLGGSRYLVAEADESDA
SFLHLTPVISVVTNIEADHMDTYGGDVEKLKQTFVDFLHNLPFYGVAVMCVDDGYVQEII
PRISRAIITYGIDNPEADYRAESINSDGLRTHFVVKRPAGRSDLTVELKMPGRHNVLNAL
AAIAVATDEGVADDAICRGLAGFAGVGRRFQVYGDYQTPKGTVTLVDDYGHHPTEVEAVI
RAAHDAWPDRRLVMLYQPHRFTRTRDLYEDFVRVLSEVDGLLLMDVYSAGEPAIPGADGR
ALCRSIRQRGQVEPVFVEDNLEIESLLANVLQDGDLLITQGAGDIGGVAARLAAAGVIAG
E