Protein Info for Psest_0566 in Pseudomonas stutzeri RCH2

Updated annotation (from data): urea ABC transporter, permease component
Rationale: Specific phenotype: utilization of Parabanic Acid. Parabanic acid is probably hydrolyzed to urea before uptake
Original annotation: urea ABC transporter, permease protein UrtB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 234 to 258 (25 residues), see Phobius details amino acids 265 to 284 (20 residues), see Phobius details amino acids 290 to 310 (21 residues), see Phobius details amino acids 323 to 344 (22 residues), see Phobius details amino acids 371 to 391 (21 residues), see Phobius details amino acids 423 to 442 (20 residues), see Phobius details amino acids 455 to 481 (27 residues), see Phobius details amino acids 489 to 508 (20 residues), see Phobius details TIGR03409: urea ABC transporter, permease protein UrtB" amino acids 229 to 520 (292 residues), 436.1 bits, see alignment E=3.8e-135 PF02653: BPD_transp_2" amino acids 232 to 506 (275 residues), 150.1 bits, see alignment E=7.1e-48

Best Hits

KEGG orthology group: K01997, branched-chain amino acid transport system permease protein (inferred from 96% identity to psa:PST_3721)

Predicted SEED Role

"Urea ABC transporter, permease protein UrtB" in subsystem Urea decomposition

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GEL4 at UniProt or InterPro

Protein Sequence (523 amino acids)

>Psest_0566 urea ABC transporter, permease component (Pseudomonas stutzeri RCH2)
MNTALHRLLLLLLLALPWAAQAGDAADYAKASPAKQARLLEDWAIAPVPERLPLIEALRA
NRVAIDQNKTPFVEDGDTYRALESDAEPVGTPKKLRLNNRLRGLLNTALASHQLFSEDPS
QRLGAAKLLQRSGQPEQLPLLQQRLEAEDDPAAREALTLALANLQLTASDPAVRLAAVRL
LGETGAPLARVRLENLLADEAETDAAVRKAAETSLAQVKRKLLIGELLGQAFSGLSLGSI
LLLAALGLAITFGLLGVINMAHGEMLMLGAYTTYMVQVLMARLAPEALAFYPLVALPVAF
FVTAAIGMALERTVIRHLYGRPLETLLATWGISLILIQLVRVLFGAQNVEVANPAWLSGG
LQVLPNLVLPYNRMVIIGFALFVVLLTWLLLNKTRLGLNVRAVTQNRNMAACCGVPTGRI
DMLAFGLGSGIAGLGGVALSQIGNVGPDLGQSYIIDSFLVVVLGGVGQLAGSIFAAFGLG
IANKILEPQIGAVLGKILILALIILFIQKRPQGLFALKGRVID