Protein Info for PGA1_c00550 in Phaeobacter inhibens DSM 17395

Annotation: putative cob(II)yrinic acid a,c-diamide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 transmembrane" amino acids 137 to 159 (23 residues), see Phobius details TIGR02476: 5,6-dimethylbenzimidazole synthase" amino acids 8 to 207 (200 residues), 284.9 bits, see alignment E=1.4e-89 PF00881: Nitroreductase" amino acids 21 to 186 (166 residues), 93.2 bits, see alignment E=1.1e-30

Best Hits

Swiss-Prot: 52% identical to BLUB_RHORT: 5,6-dimethylbenzimidazole synthase (Rru_A3536) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K04719, 5,6-dimethylbenzimidazole synthase [EC: 1.14.99.40] (inferred from 78% identity to sit:TM1040_2608)

MetaCyc: 37% identical to aerobic 5,6-dimethylbenzimidazole synthase (Sinorhizobium meliloti)
RXN-8771 [EC: 1.13.11.79]

Predicted SEED Role

"Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.79 or 1.14.99.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EI82 at UniProt or InterPro

Protein Sequence (213 amino acids)

>PGA1_c00550 putative cob(II)yrinic acid a,c-diamide reductase (Phaeobacter inhibens DSM 17395)
MNMRDNSFSEDFRMQLDLLMRVRRDVRRFRSDPVDEAVLTRCLDTIQLAPSVGLSEPWRV
IRVESDSARAAALENFETANATALAGFSEAKAERYAKLKLSGMREAPVQLAIYCDDSTTK
GHKLGAGTMPEMRRYSVVTAVTLFWLALRAEGLGLGWVSILDPQQLNRDLDVPADWQLIG
YFCVGHPERMSKTPELEQLGWETRRNALPIETR