Protein Info for HP15_533 in Marinobacter adhaerens HP15

Annotation: ketol-acid reductoisomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02826: 2-Hacid_dh_C
  • amino acids 13 to 83 (71 residues), 27.8 bits, E=3e-10
• PF07991: IlvN
  • amino acids 14 to 177 (164 residues), 244 bits, E=1.2e-76
• TIGR00465: ketol-acid reductoisomerase
  • amino acids 14 to 326 (313 residues), 417.9 bits, E=1.1e-129
• PF03807: F420_oxidored
  • amino acids 18 to 91 (74 residues), 28.3 bits, E=4.4e-10
• PF01450: IlvC
  • amino acids 183 to 326 (144 residues), 204.3 bits, E=2.1e-64

Best Hits

Swiss-Prot: 93% identical to ILVC_MARHV: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 93% identity to maq:Maqu_0884)

MetaCyc: 66% identical to acetohydroxyacid isomeroreductase (Cupriavidus necator H16)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of coenzyme A biosynthesis I (bacteria) (8/9 steps found)
• L-valine biosynthesis (4/4 steps found)
• phosphopantothenate biosynthesis I (4/4 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
• superpathway of L-threonine metabolism (12/18 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.86

Sequence Analysis Tools

See E4PNH9 at UniProt or InterPro

Protein Sequence (338 amino acids)

>HP15_533 ketol-acid reductoisomerase (Marinobacter adhaerens HP15)
MQVYYDKDCDLSIIQGKKVAIIGFGSQGHAHACNLKESGVDVTVGLRPGSSSIAKAEAYG
LKTSDVPSAVAAADVVMVLTPDEYQAQLYKAEIEPNLQQGATLAFAHGFAIHYNQIVPRK
DLDVIMIAPKAPGHTVRTEFANGGGIPDLIAIFQDSSGNAKNLALSYASGIGGGRTGIIE
TTFKDETETDLFGEQAVLCGGTVELVKAAFETLVEGGYEPEMAYFECLHELKLIVDLMYE
GGIANMNYSISNNAEYGEYVTGPEIINEQSREAMRNALKRIQSGEYAKMFISEGALNYPS
MTARRRQNAAHEIEVVGEKLRGMMPWISANKIVDKDKN