Protein Info for Psest_0542 in Pseudomonas stutzeri RCH2

Annotation: AMP nucleosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 491 TIGR01717: AMP nucleosidase" amino acids 16 to 491 (476 residues), 780.7 bits, see alignment E=2.8e-239 PF10423: AMNp_N" amino acids 17 to 173 (157 residues), 160.2 bits, see alignment E=3.2e-51 PF01048: PNP_UDP_1" amino acids 276 to 461 (186 residues), 68.2 bits, see alignment E=7.1e-23

Best Hits

KEGG orthology group: K01241, AMP nucleosidase [EC: 3.2.2.4] (inferred from 96% identity to psa:PST_3742)

Predicted SEED Role

"AMP nucleosidase (EC 3.2.2.4)" in subsystem Purine conversions (EC 3.2.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIL0 at UniProt or InterPro

Protein Sequence (491 amino acids)

>Psest_0542 AMP nucleosidase (Pseudomonas stutzeri RCH2)
MTSNESRSFTVATRAEEAVDKLAELHAQATAALNQALKRYVTSRTEPSAAERNLFRYPQL
RLTYECHGEVPASTRAYAKVQAPGVYSVTVTHPAAFRAYLLDQLKPLIQDFNVTVEVGMS
DRNIPYPYVIEQGDELAGTGVTAAELARVFPSTDLSAATDDIADGLYDWEHADPYPLALF
DGARVDFSLRRLVHYTGSDWRHVQPWILLTNYHRYVDQFIRHGLDMLRDDTRFTRMVLPG
NVIIERGMEEGEAQAIIGGVMWHRYQMPAYHLIADDGHGITLVNIGVGPSNAKNITDHLA
VLRPHCWLMIGHCGGLRQSQSIGDYVLAHAYMRRDGILDRVLPPHIPLPALAEVQQALQE
SAATVTGEQGEALKKRLRTGTVLTYDDRNWELRWAQERPLINLSRAVAVDMESGTIAAQG
YRLRVPYGTLLCVSDKPLHSEIKLPGSANAFYERAVTQHLKIGITALELLRSQLNSLHSR
KLRSFDEPPFR