Protein Info for HP15_525 in Marinobacter adhaerens HP15

Annotation: NAD+ synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 5 to 232 (228 residues), 87 bits, E=1.4e-28
• PF02540: NAD_synthase
  • amino acids 261 to 511 (251 residues), 244.1 bits, E=1.2e-76
• TIGR00552: NAD+ synthetase
  • amino acids 266 to 513 (248 residues), 201.4 bits, E=7.1e-64

Best Hits

Swiss-Prot: 49% identical to NADE_XYLFA: Glutamine-dependent NAD(+) synthetase (nadE) from Xylella fastidiosa (strain 9a5c)

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 88% identity to maq:Maqu_0876)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (6/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (4/7 steps found)
• NAD salvage pathway V (PNC V cycle) (2/5 steps found)
• NAD de novo biosynthesis II (from tryptophan) (4/9 steps found)
• NAD salvage (plants) (4/11 steps found)
• superpathway of NAD biosynthesis in eukaryotes (4/14 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Sequence Analysis Tools

See E4PNH1 at UniProt or InterPro

Protein Sequence (538 amino acids)

>HP15_525 NAD+ synthetase (Marinobacter adhaerens HP15)
MVMAQLDFLVGDIPGNTELVLDAARRASSEHQADVVVFPELCLTGYPPEDLLLRPSMEVR
VNEALERLQAERLDPVIVIGAPLRHGALLYNAAVVIDAGEITGRYFKRFPPNYQVFDEKR
YFAEGRDVLTLDIRGVPVGITVCEDLWKEGPVEDCAAAGARLILNLNASPYDIDKQARRK
ALLERKSRENLVSIVYVNLVGGQDELVFDGGSMVFDHSGVLTAEAPQFGEGLYPVDFLCE
HHCQPVSQALPEEPTLEANVYRALVTGVRDYVNKNGFKSVVLGLSGGIDSAVTLAVAVDA
LGKDRVRAVMMPFRYTSSMSLEDAEAEAVALGVQYDVFSIEPMYDAFMETLAGPFEGTTP
DTTEENLQARLRGVLLMSLSNKFGSLVLTTGNKSEMAVGYSTLYGDMAGGFDVLKDVPKT
LVFRLAKYRNTLSEGEVIPERVITRPPSAELAPDQKDEDSLPGYDVLDQILNLYVERDFS
ADAIVAEGFERVDVERVIRLVDINEYKRRQAPIGVRITERGFGKDRRYPITNGWKSGK