Protein Info for HP15_512 in Marinobacter adhaerens HP15

Annotation: isoleucyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 939 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 845 (831 residues), 946.5 bits, see alignment E=7.4e-289 PF00133: tRNA-synt_1" amino acids 28 to 643 (616 residues), 486.6 bits, see alignment E=1.7e-149 PF09334: tRNA-synt_1g" amino acids 58 to 109 (52 residues), 34.5 bits, see alignment 3e-12 amino acids 585 to 635 (51 residues), 21.1 bits, see alignment 3.7e-08 PF13603: tRNA-synt_1_2" amino acids 305 to 367 (63 residues), 30.4 bits, see alignment 8.1e-11 PF08264: Anticodon_1" amino acids 688 to 844 (157 residues), 96.8 bits, see alignment E=3.5e-31 PF06827: zf-FPG_IleRS" amino acids 901 to 927 (27 residues), 32.6 bits, see alignment (E = 1.7e-11)

Best Hits

Swiss-Prot: 62% identical to SYI_ALCBS: Isoleucine--tRNA ligase (ileS) from Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 62% identity to abo:ABO_0459)

MetaCyc: 60% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PNF8 at UniProt or InterPro

Protein Sequence (939 amino acids)

>HP15_512 isoleucyl-tRNA synthetase (Marinobacter adhaerens HP15)
MSDYKHTLNLPETAFPMRGNLAKREPEMLKRWQDLDVYGNLRKQREGREKFILHDGPPYA
NGSIHIGHAVNKILKDMIVKSRSFMGYDAPYVPGWDCHGLPIEHKVEQEIGKAGVKVDYK
TFRQACRDYATKQIAGQKTDFIRLGVMGEWDKPYLTMDPKVEAGIVRALGKIVAKGHLVR
GYKPVYWSVVGQSALAEAEVEYQDKTSTQIDVRFTAVDQAKALSLFGTDNGNGDVSVVIW
TTTPWTIPANQAVSLNADLDYALVQTDVGHGPERMILAADMVDGIMARWQVESYEVLATC
AGAALENLVLQHPIYDKQVPVILGDHVSTDAGTGAVHTAPDHGMEDFEVGKAYNIGTINL
VQADGTYTSAAGELAGVHVYKADEPVCSALEREGKLVRSEKFRHSYPHCWRTKTPLIYRA
TPQWFISMDKENLRADALEAIQGVRWVPSWGKNRIEAMFNQSPDWCISRQRTWGVPITLF
IHKETQELHPDTQNLIEQVAKQIEEGGIDAWYELDASSLLGSDAEQYEKVTDTLDVWFDS
GVTHESVLRVREELGQFPADMYLEGSDQHRGWFQSSLKTSIAMNGVAPYRQVLTHGFTVD
GKGHKMSKSLGNVIAPQEVMNELGADILRLWVAATDYSGEMTVSKDILRQTADGYRRIRN
TSRFLLSNLTGFDPEQHMVAPEDMIALDRWMVDRALQLQEELDEDYGNYAFLRIYQKVYN
FCEATLGGFYLDIIKDRQYTTQADSLARRSCQTALYHVAEALVRWIAPILSFTADEIWQH
LPGKRSDTVFYETWYEGLTALPDNAELGRDYWREMYSVKEAVNKCLEEARARGEIKGSLS
AEVTLFCEGDLAADLKHLGEELRFVLITSEASVKPVSEAGDAEMTSHEGLRVKVTPAAHT
KCERCWHHREDVGRNAKYDDLCGRCVTNVEGPGEARAYA