Protein Info for PGA1_c05140 in Phaeobacter inhibens DSM 17395

Annotation: D-amino acid dehydrogenase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 416 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01266: DAO" amino acids 5 to 398 (394 residues), 203.3 bits, see alignment E=4.2e-64

Best Hits

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 66% identity to sil:SPOA0262)

Predicted SEED Role

"D-amino acid dehydrogenase (EC 1.4.99.1) family protein in hydroxy-L-proline catabolic cluster" in subsystem Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DM85 at UniProt or InterPro

Protein Sequence (416 amino acids)

>PGA1_c05140 D-amino acid dehydrogenase small subunit (Phaeobacter inhibens DSM 17395)
MTKTVVVIGAGVVGVSAALRLQGELAPAGGQVILLDRKGVAEETSAGNAGAFAFTDLEPL
ATPGIMKKAPAWLLDPLGPLSVPPAYALQLTPWLIRFWRASWRDRYQKVVSAQASLMQLA
QAATERLITDTKGEAMMQRDGALQLYESEGEFAASQRGWQLRRDHGIRFTLLHSPAEIAE
IQPGLSPRFTHAGFTPDWMNTCDPKAWVEHLFQIFMARGGQLRRAEVRDLETGGEDLCLL
TDGESVRANNVVVAAGAWSHHLTRGLGDRLPLETERGYNTTLPAGCFDLRTHITFCRHGF
VVSRIRGGVRVGGAVELGGLKRPANFKRADTLLAKAARFMPELDTANGTRWMGFRPSLPD
SLPVIGNAKSDGRVVYAFGHGHVGLTQSAATAELVADLVLRRDPAIDLSPFAPDRF