Protein Info for Psest_0005 in Pseudomonas stutzeri RCH2

Annotation: DNA gyrase, B subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 TIGR01059: DNA gyrase, B subunit" amino acids 7 to 803 (797 residues), 997.7 bits, see alignment E=1.2e-304 PF02518: HATPase_c" amino acids 34 to 172 (139 residues), 66 bits, see alignment E=1.2e-21 PF00204: DNA_gyraseB" amino acids 221 to 390 (170 residues), 194.7 bits, see alignment E=2.7e-61 PF01751: Toprim" amino acids 419 to 531 (113 residues), 68.3 bits, see alignment E=1.6e-22 PF21249: GyrB_hook" amino acids 533 to 561 (29 residues), 56.5 bits, see alignment (E = 4.8e-19) PF18053: GyrB_insert" amino acids 564 to 730 (167 residues), 185.4 bits, see alignment E=2.4e-58 PF00986: DNA_gyraseB_C" amino acids 734 to 793 (60 residues), 105.4 bits, see alignment 4.1e-34

Best Hits

Swiss-Prot: 88% identical to GYRB_PSEAE: DNA gyrase subunit B (gyrB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 88% identity to avn:Avin_00040)

MetaCyc: 69% identical to DNA gyrase subunit B (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GD28 at UniProt or InterPro

Protein Sequence (804 amino acids)

>Psest_0005 DNA gyrase, B subunit (Pseudomonas stutzeri RCH2)
MSENHTYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNSIDEALAGHCSD
ISITIHTDESITVRDNGRGIPVDIHEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVG
VSVVNALSEELMLTIRREGKVWEQLYRHGVPQAPLAPVGETDSSGTQIHFKPSAETFQNI
HFSWDILAKRLRELSFLNSGVGIVLRDERTAKEELFKYEGGLSAFVAYLNTNKTPVNEVF
HFNVQRDDGVGVEVALQWNDSFNENILCFTNNIPQRDGGTHLAGFRSALTRNLNSYIEQE
GLAKKHKIATTGDDAREGLTAIISVKVPDPKFSSQTKDKLVSSEVKTAVEQEMGKHFADF
LLEHPSEAKAVVGKMIDAARAREAARKAREMTRRKGALDIAGLPGKLADCQEKDPALSEL
YIVEGDSAGGSAKQGRNRKTQAILPLKGKILNVEKARFDKMISSQEVGTLITALGCGIGR
DEYNIDKLRYHNIIIMTDADVDGSHIRTLLLTFFFRQLPELIERGYVYIAQPPLYKVKRG
KQEQYIKDDEAMEEYLTQSALEDASLHVNEGAPGLSGGALEKLVNDYRTVMKTLQRLSRL
YPQELTEHFIYLPRVPVEQLADHSAMQAWLEQYSALLKVGERSGLIYNVSLREDKERHLW
LPEVEISSHGLASYITFNRDFFGSNDYRTVTALGEKLNSLLEEGAYVQRGERKKQVATFK
QALEWLMTEGTRRHSVQRYKGLGEMNPDQLWETTMDPNVRRMLKVTIEDAIGADQIFNTL
MGDAVEPRRDFIETNALAVSNLDF