Protein Info for PGA1_c05020 in Phaeobacter inhibens DSM 17395

Annotation: phenylacetic acid degradation NADH oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 361 PF00970: FAD_binding_6" amino acids 29 to 117 (89 residues), 27.3 bits, see alignment E=5.9e-10 PF00175: NAD_binding_1" amino acids 129 to 235 (107 residues), 51.2 bits, see alignment E=2.7e-17 PF00111: Fer2" amino acids 285 to 352 (68 residues), 49.1 bits, see alignment E=6.6e-17

Best Hits

KEGG orthology group: None (inferred from 79% identity to sit:TM1040_3218)

MetaCyc: 66% identical to stachydrine N-demethylase reductase subunit (Sinorhizobium meliloti 1021)
R501-RXN [EC: 1.14.13.247]

Predicted SEED Role

"Flavodoxin reductases (ferredoxin-NADPH reductases) family 1" in subsystem Anaerobic respiratory reductases

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.13.247

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DM75 at UniProt or InterPro

Protein Sequence (361 amino acids)

>PGA1_c05020 phenylacetic acid degradation NADH oxidoreductase (Phaeobacter inhibens DSM 17395)
MNELSQKSAPAIWSDSELLECVSVVPEMPNTASFSFRAPSGALFAYDPGQFLTLEIPALD
QPGGMVHRTYTISSSPSRPRSITITAKAQPDSIGTRWMLDNLKPGMTIRAIGPAGLFSNA
GSRARKYLFISAGSGITPMMSMTTCMWDEGGNLDVVFINCASRPSEIIFRQRLEQMASRT
PGLDLKFVVEEPDSYRPWTGYQGYFNQLMLGLMAPDYLDREVYCCGPEPFMQAVREALTG
LGFDMENYHQESFGAPVATEADAPDLDDVVPDSATTAELHFSLSDVTHKCAETDTVLGAA
KAAGLNIPSGCTFGVCGTCKVKKTEGEVHMVHNGGISEDDIEAGYILACCSNPIGSVSVE
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