Protein Info for Psest_0494 in Pseudomonas stutzeri RCH2

Annotation: Rhodanese-related sulfurtransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00581: Rhodanese
  • amino acids 13 to 122 (110 residues), 52.8 bits, E=2.4e-18
  • amino acids 152 to 262 (111 residues), 61.9 bits, E=3.3e-21

Best Hits

Swiss-Prot: 78% identical to THTR_AZOVI: Thiosulfate sulfurtransferase (rhdA) from Azotobacter vinelandii

KEGG orthology group: K01011, thiosulfate/3-mercaptopyruvate sulfurtransferase [EC: 2.8.1.1 2.8.1.2] (inferred from 89% identity to psa:PST_3799)

MetaCyc: 78% identical to thiosulfate sulfurtransferase (Azotobacter vinelandii)
Thiosulfate sulfurtransferase. [EC: 2.8.1.1]

Predicted SEED Role

"Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1)" (EC 2.8.1.1)

MetaCyc Pathways

• thiosulfate disproportionation IV (rhodanese) (1/1 steps found)
• L-cysteine degradation III (1/2 steps found)
• sulfide oxidation IV (mitochondria) (2/5 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.1, 2.8.1.2

Use Curated BLAST to search for 2.8.1.1 or 2.8.1.2

Sequence Analysis Tools

See L0GGH2 at UniProt or InterPro

Protein Sequence (271 amino acids)

>Psest_0494 Rhodanese-related sulfurtransferase (Pseudomonas stutzeri RCH2)
MSAFSNLPLVIEPADLAPRLDAPELILVDLTSASRYAEGHLPGARFVDPKQTQLGQPPAP
GLLPPQAQLERLFGALGHRADAVYVVYDDEGGGWAGRFIWLLDVIGHHRYHYLNGGLRAW
LEEARPLTQEVPAAASGSPSLALDDAPTATRTYIESRLGAEDLVIWDARSPAEYRGEKLF
AARGGHIPGAINFEWTAAMDPARALRIREDIAEQLQALGIVAEKEIITHCQTHHRSGLTY
LLAKALGYPRVKGYAGSWSEWGNLPDTPVQQ